Autoantigen administration confers diabetes-preventive properties to NOD mice derived dendritic cells  

作　　者：MING XIANG XIAO LEI ZOU JING XU YAO YAO JIN FANG ZHANG 

机构地区：[1]Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong Science and Technology University, Wuhan, P. R. China

出　　处：《Journal of Microbiology and Immunology》2007年第1期69-77,共9页中华微生物学和免疫学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(No.30200343).

摘　　要：Administration of autoantigen can be of value for prevention of autoimmune diabetes and it has been speculated that the control point of dendritic cells(DC)for the induction of peripheral toler- ance may be highly relevant.We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and their ability to suppress diabetes transfer by diabeto- genic effector cells in secondary NOD-SCID recipients.Our data showed that the surface expressions of MHCⅡand CD86 on NOD-derived DC were increased after insulin treatment compared with those on PBS controlled mice.The dendritic cells with a mature phenotype and increased MLR stimulation adop- tively transferred immune tolerogenic effects on secondary NOD-SCID mice,which were associated with significantly greater IL-10,TGF-beta production and CD4^+ CD25^+ T differentiation from splenocytes compared with NOD-SCID control recipients.Moreover,treatment with DC remarkably decreased the incidence of diabetes in secondary recipients.These results suggest that a subtype of DC generated by insulin subcutaneous treated NOD mice confers potential protection against diabetes through polarizing the immune response towards a Th2 regulatory pathway.Administration of autoantigen can be of value for prevention of autoimmune diabetes and it has been speculated that the control point of dendritic cells （DC） for the induction of peripheral tolerance may be highly relevant. We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and their ability to suppress diabetes transfer by diabetogenic effector cells in secondary NOD-SCID recipients. Our data showed that the surface expressions of MHC Ⅱ and CD86 on NOD-derived DC were increased after insulin treatment compared with those on PBS controlled mice. The dendritic cells with a mature phenotype and increased MLR stimulation adoptively transferred immune tolerogenic effects on secondary NOD-SCID mice, which were associated with significantly greater IL-10, TGF-beta production and CD4 ^＋ CD25 ^＋ T differentiation from splenocytes compared with NOD-SCID control recipients. Moreover, treatment with DC remarkably decreased the incidence of diabetes in secondary recipients. These results suggest that a subtype of DC generated by insulin subcutaneous treated NOD mice confers potential protection against diabetes through polarizing the immune response towards a Th2 regulatory pathway.

关 键 词：Diabetes Dendritic cells Immune tolerance NOD mice 

分 类 号：R392.3[医药卫生—免疫学]