硫化镉纳米微粒与某些氨基糖苷类抗生素相互作用的共振瑞利散射光谱及其分析应用  

Resonance Rayleigh Scattering Spectra of Aminoglycoside Antibiotics with CdS Nanoparticles and Their Applications in Analyses

作　　者：王齐[1] 刘忠芳[1] 刘绍璞[1] 胡小莉[1] 

机构地区：[1]西南大学 化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2007年第7期64-68,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金(20475045).

摘　　要：在pH为2.0～7.0的水溶液中,硫化镉纳米微粒[(CdS)_n]与氨基糖苷类抗生素阿米卡星(AMK)、卡那霉素(KANA)、妥布霉素(TOB)和庆大霉素(GEN)借静电引力、疏水作用力结合,形成粒径更大的聚集体,导致共振瑞利散射(RRS)的增强并产生新的RRS光谱,最大RRS峰位于289 nm(AMK体系)、291 nm(KANA和TOP体系)和287 nm(GEN体系).在一定条件下,RRS散射强度(ΔI)均与药物的浓度成正比,反应具有高灵敏度,对于4种药物的检出限在7.7～8.8 ng/mL之间.其中(CdS)_n-AMK体系灵敏度最高,对AMK的检出限为7.7 ng/mL.据此发展了一种用纳米硫化镉作探针,灵敏、简便、快速测定氨基糖苷类抗生素药物的共振瑞利散射新方法.In pH=2.0-7.0 water solution, the CdS nanoparticles [（CdS）n] can react with some kinds of aminoglycoside antibiotics （AMK, KANA, TOB and GEN） to form bigger aggregates by electrostatic attraction and hydrophobic force. The aggregates greatly arouse Resonance Rayleigh scattering （RRS）, and new RRS spectra appear. The maximum RRS peaks are located at 289 nm for AMK system, 291 nm for KANA system, 291 nm for TOB system and 287 nm for GEN system. The enhancements of scattering （△I） are directly proportional to the concentration of the aminoglycoside antibiotics. The reactions have high sensitivities, and the detection licnits for the 4 different drugs studied are 7.7-8.8 ng/mL. Among them, （CdS）n-AMK system is the most sensitive, and its limits for AMK are 7.7 ng/mL. So a new method for the determination of aminoglycoside antibiotics with CdS nanoparticles as probe has been developed. The method is simple and fast, and highly sensitive.

关 键 词：硫化镉 纳米微粒 共振瑞利散射 氨基糖苷类抗生素药物 

分 类 号：O657.3[理学—分析化学]