SARS病毒E基因的克隆与重组腺病毒rAdE-E的构建  

Cloning of SARS-CoV E Gene and Construction of Its Recombinant Adenovirus rAdE-E

作　　者：刘正学[1] 齐义鹏[2] 

机构地区：[1]重庆三峡学院生物系,重庆万州404000 [2]武汉大学生命科学学院病毒学国家重点实验室,武汉430072

出　　处：《西南大学学报（自然科学版）》2007年第7期130-134,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家"973"资助项目(2003CB514120).

摘　　要：为了进一步研究SARS病毒E蛋白的功能以及在SARS致病机理中的作用,我们通过touch-down PCR程序,从SARS病毒WHU株(GenBank accession number AY394850.1)cDNA文库中扩增了E基因,并通过基因工程技术及腺病毒系统构建了重组腺病毒rAdE-E.DNA测序、酶切鉴定及荧光检测(GFP表达)结果均表明:重组腺病毒rAdE-E构建成功.To further explore the function of SARS-CoV small envelope protein （E） and its role in virus pathogenesis, the gene fragment encoding E protein was amplified from the SARS-CoV WHU （GenBank accession number AY394850.1） cDNA library by touch-down PCR, and the recombinant adenovirus rAdE- E was constructed by genetic engineering technology and AdEasy system. The results suggested that the rAdE-E was successfully constructed, as was shown by DNA sequencing, restriction endonuclease digestion analysis and fluorescence detection （GFP expression）.

关 键 词：SARS病毒(SARS-CoV) E蛋白 腺病毒系统 细胞凋亡 

分 类 号：Q78[生物学—分子生物学]