基因药物载体重组古菌组蛋白的免疫原性研究  

Immunogenicity of the recombinant archaeal histone as a non-viral gene delivery

作　　者：李媛媛[1] 王蕊[1] 郑艳军[2] 张国利[2] 朱平[2] 牛俊奇[3] 冯雁[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130023 [2]军事医学科学院军事兽医研究所,长春130062 [3]吉林大学第一医院感染科,长春130021

出　　处：《免疫学杂志》2007年第5期474-477,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30571642)

摘　　要：目的观察基因药物载体重组古菌组蛋白(HphA)在鼠模型中的免疫原性,为开发安全有效的基因药物载体重组古菌组蛋白奠定基础。方法Balb/c小鼠随机分为重组古菌组蛋白实验组、重组古菌组蛋白佐剂实验组、牛血清白蛋白佐剂对照组,测定免疫小鼠中体液和细胞免疫应答水平。体液免疫应答水平通过采用酶联免疫吸附试验(ELISA)考察测定血清中的抗体水平;细胞免疫应答水平通过测定脾淋巴细胞的增殖指数和其分泌的IFN-γ浓度进行评价。结果ELISA法未检测出重组古菌组蛋白免疫小鼠血清中产生特异性抗体,淋巴细胞的刺激指数及其分泌IFN-γ浓度与对照组相比无统计学意义(P>0.05)。结论重组古菌组蛋白免疫在鼠模型中没有免疫原性,不能诱导免疫小鼠产生特异性体液应答和细胞免疫应答。Objective To investigate the immunogenicity of the recombinant archaeal histone （HphA） as a non-viral gene delivery in Balb/c mice, for providing a base for safe and effective application of HphA. Methods Mice were divided randomly into 3 groups, and then immtmized by HphA, HphA combining with Freuds adjuvant, and bovine serum albumin combining with Freuds adjuvant, respectively. Humoral immunoresponse was evaluated with the serum IgG level tested by ELISA, while cellular immunologic response was evaluated with stimulation indexes and levels of IFN-γ secreted from splenocytes. Results Mice immunized with HphA produced no specific ant/body, and the stimulation indexes and levels of IFN-γ in HphA-immunized mice were no difference from those of the control group. Condusion The HphA had no immunogenicity in mouse, and could not produce humoral and cellular immune response.

关 键 词：重组古菌组蛋白 抗体 T淋巴细胞 免疫原性 

分 类 号：R392[医药卫生—免疫学]