牛FABGL基因的分离与染色体RH定位  

Study on Isolating,RH Mapping of Bovine FABGL Gene

作　　者：马云[1] 高雪[1] 任红艳[1] 许尚忠[1] 张英汉[2] 辛亚平[2] 高树新[3] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094 [2]西北农林科技大学动物科技学院,杨凌712100 [3]内蒙古民族大学动物科技学院,通辽028000

出　　处：《畜牧兽医学报》2007年第9期893-898,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："863"国家高技术研究发展计划(2002AA242011)

摘　　要：以中国西门塔尔牛血样为材料,提取基因DNA,运用同源序列克隆技术对牛FABGL基因DNA序列进行了克隆与分析,应用SUNbRH7000型辐射杂种板(RH)对分离的牛FABGL基因进行了染色体定位。结果显示,本研究所获得的长为1 925 bp的DNA序列为牛FABGL基因的包含全部9个外显子的基因组序列。该序列与人(NM-014234)和猪(AF-146398)的FABGL基因序列的相似性分别为84%和90%;牛FABGL基因被定位于牛的23号染色体上,与该染色体上的标记BM47的距离为1.71 cR,两点评分值为31.05。The blood samples from Chinese Simmental cattle was collected to extracted genomic DNA and the DNA of the bovine FABGL gene was determined and analyzed by homology cloning approach combined with PCR in this study. Sequence analysis and bioinformatics study showed that this genomic DNA contained 1925 nucleotides and 9 exons. And its base composition shows 90% and 84% identity with the corresponding pig and human, respectively. The SUNbRH panel was employed to determined the precise location of FABGL gene. Statistical analysis revealed that the bovine FABGL gene was mapped to the bovine chromosome （BTA） 23 and was closely linked to a framework marker BM47 at a 2-point LOD score of 31.05.

关 键 词：牛 β-酮酰基还原酶基因 分离 辐射杂种板 定位 

分 类 号：S823.2[农业科学—畜牧学]