用间接免疫荧光染色法检测鸭病毒性肠炎病毒在人工感染鸭体内的侵染过程和分布规律  

Research on the Invading Process and Distribution of Duck Enteritis Virus in Artificially Infected Ducks by Indirect Immuno-Fluorescent Staining Method

作　　者：程安春[1] 韩晓英[1] 汪铭书[1] 袁桂萍[1] 徐超[1] 廖永洪[1] 

机构地区：[1]四川农业大学动物医学院禽病防治研究中心

出　　处：《畜牧兽医学报》2007年第9期942-946,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(3047129);教育部"新世纪优秀人才支持计划"项目(NCET-04-0906);高等学校科技创新工程重大项目培育资金项目(706050);国家农业科技成果转化资金(2006GB2F00249);四川省重点建设学科项目(SZD0418)

摘　　要：用鸭病毒性肠炎病毒(DEV)CHv强毒株感染成年鸭复制鸭病毒性肠炎急性病例,分别于接种后不同时间,取心、肝、脾、肺、肾、胸腺、食道、十二指肠、胰腺、法氏囊和脑组织,制作切片,应用间接免疫荧光染色法(IFA)检测DEV在鸭体内的侵染过程和分布规律。结果显示:感染后4 h可在脾脏、胸腺和法氏囊中检测到DEV抗原;感染后6 h可在肝脏、食道、十二指肠、直肠及肺脏检测到DEV抗原;IFA对各组织器官中DEV的平均检出率为肝脏46/50、脾脏48/50、肺脏46/50、肾脏0/50、肠道46/50、法氏囊46/50、胸腺47/50、胰腺0/50、大脑0/50、食道44/50、心脏0/50。研究表明:在急性病例中,脾脏、法氏囊、胸腺、食道、肠道、肝脏和肺脏为DEV的主要靶器官;接种后,病毒首先在脾脏、胸腺、法氏囊中出现,然后病毒迅速传播到肝脏、消化道和肺脏中;IFA检测石蜡切片中DEV的方法具有直观、特异性强的优点,是对DEV进行检测和抗原定位的较好方法。Fifty adult ducks were inoculated with a virulence duck enteritis virus（DEV, strain CHv）, and acute cases of duck viral enteritis（DVE） were duplicated. Tissue samples from heart, liver, spleen, lung, kidney, thymus, duodenum, bursa of Fabricius, brain, esophagus and the pancreas were collected after inoculation and paraffin slices of these samples were prepared. The method of indirect immuno-fluorescent staining （IFA） was applied to investigate the invading process and distribution of DEV. The results showed that DEV antigen can be detected in the spleen, thymus and bursa of Fabricius 4 hours after infection and in the liver, esophagus, duodenum, rectum and the lung 6 hours after infection. The positive rate of viral antigen in different tissues detected by IFA was 46/50 in the liver, 48/50 in the spleen, 46/50 in the lung, 0/50 in the kidney, 46/50 in the intestine, 46/50 in the bursa of Fabricius, 47/50 in the thymus, 0/50 in the pancreas, 0/50 in the brain, 44/50 in the esophagus and 0/50 in the heart. The research suggested that the spleen, bursa of Fabricius, thymus, esophagus, intestine, liver and the lung are the main target organs of DEV in acute DVE cases. After infection, DEV appears in the spleen, the thymus and bursa of Fabricius first, then spreads to the liver, alimentary canal and the lung quickly. IFA is a sensitive and specific method in detecting DEV antigen in paraffin slices and it is a good method for diagnosis and antigen location of DEV.

关 键 词：间接免疫荧光染色 鸭病毒性肠炎病毒 检测 分布 

分 类 号：S852.659.1[农业科学—基础兽医学]