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作 者:曹静[1] 陈耀星[1] 王子旭[1] 李俊英[2] 谢电[1] 贾六军[1] 李艳红[1]
机构地区:[1]中国农业大学动物医学院,北京100094 [2]中国农业大学动物科技学院,北京100094
出 处:《畜牧兽医学报》2007年第9期972-976,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA
基 金:北京市自然科学基金资助项目(6032014);高等学校博士科学点专项科研基金(2004019002);新世纪优秀人才支持计划(NCET-04-0126)资助
摘 要:采用LED(Light-emitting diodes)灯作为光源,比较单色光对雄性AA肉鸡睾丸形态结构的影响,并对其内在机理进行探讨。将48只刚出壳的AA肉鸡分为4个处理组,试验组为红光组(660 nm)、绿光组(560 nm)和蓝光组(480 nm),对照组为白光组(400-760 nm),每组12只,自由采食。光照强度为15 lx,光照制度23 h∶1 h(L:D)。49日龄利用放射免疫法检测血清中甲状腺激素和睾酮的含量,并测量体重、睾丸重、睾丸体积、曲精细管面积和生精细胞百分数。结果表明,蓝、绿光对睾丸的影响较显著,蓝、绿光组的体重、睾丸重、睾丸体积、曲精细管面积和生精细胞百分数显著高于红、白光组7.22%-32.37%(P〈0.05),且蓝光组T3、T4和睾酮水平比其他光组高14.36%-26.20%。结果提示蓝光比其他单色光更能促进甲状腺激素和睾酮的分泌,进而影响肉鸡睾丸的形态结构。Using LED (light-emitting diodes) as light sources, AA (Arbor Acres) broiler' s morphology of testes were compared with the monochromatic light and discuss the internal mechanism. Day-old 48 male broilers were divided into four light treatments (n= 12), including red (660 nm), green (560 nm), blue (480 nm) and control white (400-760 nm) by using LED. Feed and water were provided for ad libitum consumption. Light intensity was 15 lx at the height of birds' heads and scheduled for 23 h of light and 1 h of dark during the experiment. Serum testosterone and thyroid hormone concentration were determined by using radioimmunoassay and testis weight, testis volume, seminiferous tubule area and the percentage of germ cells were measured at 49 days of age. The results showed that testes developments were affected significantly on blue and green light. Body weight, testis weight, testis volume, seminiferous tubule area and the percentage of germ cells in blue and green light groups were significant higher than those of red and white light groups (7.22% -32.37%, P〈0. 05), and thyroid hormone and testosterone level in blue were higher than those of others (14.36% -26.20%). In conclusion, blue light could be more efficient to stimulate thyroid hormone and testosterone than other monochromatic lights and to affect morphology of testis in broilers.
分 类 号:S852.16[农业科学—基础兽医学]
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