小鼠内耳显微解剖技术照明方法的改进  

The Application of Improved Illumination Method for the Microdissection of the Mice Inner Ear

作　　者：周良强[1] 褚汉启[1] 黄孝文[1] 吴振恭[1] 王燕[1] 倪坤[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉科,武汉430030

出　　处：《听力学及言语疾病杂志》2007年第5期411-412,415,I0004,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目(编号30371526和30672307)

摘　　要：目的介绍连续变倍体视显微镜(Zoom-stereo microscope)的一种特殊的暗视野照明方法在内耳显微解剖中的应用。方法在小鼠耳蜗解剖中,采用纤维导光束(也称万向冷光源)从玻璃平皿侧面照射(侧射式),使光线从侧面进入,经标本反射后进入连续变倍体视显微镜光学系统而成像;并与常规的两种照明方法(落射式和透射式)的效果进行比较。结果侧射式照明效果明显比落射式和透射式好,其背景光线的干扰显著降低,达到暗视野成像效果。结论采用侧射式照明,可以提高内耳显微解剖技术的水平。Objective To introduce a special method of illumination for the microdissection of the mice inner ear using the zoom - stereo microscope. Methods Using the fibre optic illuminatior, this would allow the light to enter from the lateral of the fiat plate, and the reflect light from the specimen would enter into the optic system of the zoom - stereo microscope. Thus the lateral illumination was compared with the traditionary illuminations, namely, the falling - illumination and the transillumination. Results The effect of the lateral illumination was better than the traditionary illuminations with less background interference. Conclusion The lateral illumination can improve the mierodissection of the inner ear.

关 键 词：内耳 体视显微镜 纤维导光束 侧射式照明 对比度 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]