高糖低脂膳食对血清磷脂和甘油三酯含量的影响及其相关因素分析  

Effects of a High-carbohydrate,Low-fat Diet on the Levels of Serum Phospholipids and Triglycerides

作　　者：汤慧[1] 李正科[1] 林佳[1] 黄鑫[1] 甘婵芬[1] 李蓉晖[1] 胡雯[2] 方定志[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041 [2]四川大学华西医院营养科

出　　处：《四川大学学报（医学版）》2007年第5期822-825,共4页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会项目(CMB;NO88-486);四川省重点科技项目(03SG022-002)资助

摘　　要：目的研究高糖低脂(HC/LF)膳食对血清磷脂(PL)及甘油三酯含量的影响,并分析其相关因素。方法56名志愿者进食7 d平衡膳食及6 d高糖低脂膳食,受试者于摄取平衡膳食第1 d、高糖低脂膳食第1 d、高糖低脂膳食结束次日分别接受体质量、腰围、臀围、上臂围、皮褶厚度(肱三头肌、肩胛下角、脐旁)的测定。同时抽空腹静脉血。采用酶法测定血清TG、TC、PL。磷钨酸-镁沉淀分离高密度脂蛋白(high density lipoprotein,HDL),酶法测定高密度脂蛋白磷脂(HDL-PL)、高密度脂蛋白甘油三酯(HDL-TG)、高密度脂蛋白胆固醇(HDL-C)。聚乙烯硫酸盐沉淀分离非低密度脂蛋白,酶法测定非低密度脂蛋白磷脂(NLDL-PL)、非低密度脂蛋白甘油三酯(NLDL-TG)、非低密度脂蛋白胆固醇(NLDL-C),计算低密度脂蛋白磷脂(LDL-PL)、低密度脂蛋白甘油三酯(LDL-TG)、低密度脂蛋白胆固醇(LDL-C)浓度。电化学发光法测定血清胰岛素浓度。结果高糖低脂膳食后血清PL、HDL-PL、LDL-PL均降低(P<0.01);TG、TRL-TG、胰岛素浓度升高(P<0.05)。相关性分析显示受试者高糖低脂膳食后血清PL含量变化(ΔPL)与BMI变化量(ΔBMI)呈明显负相关;与HDL-TG、胰岛素变化量(ΔHDL-TG、ΔInsulin)呈明显正相关。血清LDL-PL含量变化(ΔLDL-PL)与BMI、腰围变化量(ΔBMI、ΔWC)呈明显负相关。血清HDL-PL含量变化(ΔHDL-PL)与血清HDL-TG变化量(ΔHDL-TG)呈明显负相关;与血清HDL-C变化量(ΔHDL-C)呈明显正相关。血清HDL-TG含量变化(ΔHDL-TG)与受试人群腰臀比变化量(ΔW/H)呈明显正相关。结论高糖低脂膳食可诱导血清PL、HDL-PL含量减少,血清TG、胰岛素含量增加;高糖低脂膳食引起PL含量的变化与BMI变化量呈明显负相关;与HDL-TG、胰岛素变化量呈明显正相关。Objective To study the effects of a High-carbohydrate,Low-fat（HC/LF） diet on the levels of phospholipids and triglycerides in serum and analyze their association with anthropometric indexs and serum biochemical traits.Methods 56 healthy volunteers had a HC/LF diet for 6 days.The body weight,waist circumference,hip circumference,biceps circumference,and skinfold thickness were measured before and after the HC/LF diet.Venous blood was taken after fasting of 12 hours.The levers of high density lipoprotein-phospholipid（HDL-PL）,high density lipoprotein-triglyceride（HDL-TG）,high density lipoprotein-cholesterol（HDL-C） were enzymatically determined after precipitation of serum apoB containing lipoproteins with phosphotungistic acid and non-LDL-phospholipid（NLDL-PL）,non-LDL-triglyceride（NLDL-TG）,non-LDL-Cholesterol（NLDL-C） levels were enzymatically determined after precipitation of serum LDL with polyethylene sulfate.The levels of serum LDL-PL,LDL-TG,and LDL-C were calculated by minus of non-LDL from serum PL,TG,and TC levels,respectively.The level of TRL-TG was calculated by minus of HDL-TG,LDL-TG from total TG.The level of serum insulin was measured by electrochemical luminescence.Results The levels of fasting serum PL,HDL-PL and LDL-PL decreased significantly after HC/LF diet,whereas the levels of TG,HDL-TG and insulin increased.Correlation analyses indicated that after HC/LF diet the change of serum PL（ΔPL） had a negative correlation with the change of BMI（ΔBMI）,a positive correlation with the change of HDL-TG（ΔHDL-TG） and insulin（Δinsulin）.The change of serum LDL-PL（ΔLDL-PL） had a negative correlation with ΔBMI and waist circumference（ΔWC）,meanwhile the change of HDL-PL（ΔHDL-PL） had a negative correlation with ΔHDL-TG,a positive correlation with the change of HDL-C（ΔHDL-L）.A positive correlation existed between ΔHDL-TG and wais /hip circumference ratio（ΔW/H）.Conclusion HC/LF diet can decrease serum PL,HDL-PL level but increase serum levels of

关 键 词：甘油三酯 磷脂 胆固醇 胰岛素 脂蛋白 

分 类 号：R589.2[医药卫生—内分泌]