胸腺五肽三甲基壳聚糖口服纳米粒的研究  

Research on Thymopentin-loaded N-trimethyl Chitosan Nanoparticles Administered through Mouth

作　　者：唐仕炜[1] 袁晓佳[1] 张志荣[1] 宋庆国[1] 何勤[1] 

机构地区：[1]四川大学华西药学院靶向药物教育部重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2007年第5期885-888,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的制备能口服给药的胸腺五肽三甲基壳聚糖纳米粒,并观察其口服给药的药效作用。方法首先合成三甲基壳聚糖(TMC),然后采用中心组合设计优化制备工艺,制备胸腺五肽的三甲基壳聚糖口服纳米粒(Tp5-TMC-NP)。用流式细胞仪测定Wistar大鼠口服给药后对T细胞CD4+/CD8+的影响。结果采用优化工艺制备的Tp5-TMC-NP为规则圆球状,粒径范围窄,平均粒径110.6 nm,包封率78.8%。Tp5-TMC-NP经Wistar大鼠灌胃给药,流式细胞仪测定(CD4+/CD8+)/Dosage为5.65,为胸腺五肽(Tp5)直接灌胃(2.18)的2.59倍。结论口服Tp5-TMC-NP效果明显优于Tp5口服。Objective To prepare a peroral thymopentin-loaded N-trimethyl chitosan chloride-nanoparticle（Tp5-TMC-NP）,and observe the pharmacodynamic action when the Tp5-TMC-NP is taken by way of the mouth.Methods N-trimethyl chitosan chloride was first synthesized,and then Tp5-TMC-NP was prepared with the formulation technology optimized by the Central Composite Design.The influence of Tp5-TMC-NP on the ratio of CD4^＋/CD8^＋of T-lymphocytes were determined by flow cytometer.Results The regular global Tp5-TMC-NP prepared with the optimized formulation craft had the mean diameter of 110.6 nm and got the entrapment efficiency of 78.8%.The ratio of lymphocyte CD4^＋/CD8^＋ of Wistar rat administered with Tp5-TMC-NP perfusing stomach had 2.59 times higher than that with Tp5.Conclusion Taking orally the Tp5-TMC-NP has much higher efficiency than Tp5.

关 键 词：胸腺五肽 口服给药系统 纳米粒 N-三甲基壳聚糖 

分 类 号：R94[医药卫生—药剂学]