利用RAPD技术检测转绿型白化突变系W25基因组的变化  

MEASUREMENT OF GENOME CHANGES OF GREENABLE ALBINO MUTATION LINE c.v. W25

作　　者：吴殿星[1,2] 夏英武[1,2] 舒庆尧[1,2] 张耀洲[1,2] 刘贵付[1,2] 

机构地区：[1]浙江农业大学核农所 [2]浙江农业大学生化所

出　　处：《核农学报》1997年第2期89-92,共4页Journal of Nuclear Agricultural Sciences

基　　金：浙江省"8812"资助

摘　　要：转绿型白化突变系Ｗ２５系γ射线辐照籼型温敏核不育水稻２１７７ｓ获得的一个叶色突变体。在整个生育阶段，其叶色呈现白色转绿返白复绿规律变化。ＲＡＰＤ分析结果表明，７０个随机引物中有３个引物不能扩增出多态性ＤＮＡ，共扩增出４９３条ＤＮＡ条带，平均每个引物７４条，其中引物Ｈ０５能扩增出Ｗ２５和２１７７ｓ多态性产物。W25 was a greenable albino mutation line, which was derived from a temperature-sensitive genic male sterile rice 2177s, with 300Gy gamma rays irradiation. During the whole growth duration, the leaves of W25 showed the following characters: white, greenism, albinism and greenism again. 70 primers were used for the detection of polymorphism, one of them gave polymorphic products. RAPD analysis of W25 and 2177s with random primer H05 indicated that there were two DNA bands differences. \ \

关 键 词：转绿型 白化突变系 RAPD 水稻 

分 类 号：S511.035.2[农业科学—作物学] S124.1