Study on the relationship between interleukin-10 promoter polymorphism and the chronic severe hepatitis  

作　　者：DAO JIE LIU YING LIU ZHUO LI YAN YAN JI MING YIN WA HAO JIN QIN NIU FANG LIU XIAN CHUN XIE HUI LI 

机构地区：[1]Affiliated Beijing Youan Hospital of Capital University of Medical Sciences, Hepatitis Institute of BeijingMunicipal Health Bureau, Beijing, P. R. China [2]Peking Union Medical College, Beiing, P. R. China

出　　处：《Journal of Microbiology and Immunology》2007年第2期133-138,共6页中华微生物学和免疫学（英文版）

摘　　要：The aim of this study is to investigate whether three mononucleotide polymorphisms at the locus -1082,-819 and -592 in the promoter region of the IL-10 gene are associated with chronic severe hepatitis. The IL-10-592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reactionrestriction fragment length polymorphism analysis （PCR-RFLP） while polymerase chain reaction-sequence specific primer （PCR-SSP） assay was used to test the IL-10-819 polymorphism. The polymorphisms of IL-10-1082, -819 and -592 genes were detected in 98 patients with chronic severe hepatitis （CSH）, 478 patients with chronic hepatitis B （CHB）, 223 asymptomatic （chronic） HBV carriers （ASC） and 267 patients with self-restricted HBV. There was significant difference of the polymorphisms of IL-10-1082, IL-10-819 and IL-10-592 genes between CSH group and other groups. The frequency of AA genotype at IL-10 gene promoter -1082 locus in chronic severe hepatitis patients was higher than that in asymptomatic HBV carriers （2 = 13. 314, P = 0.001）, and self-restricted HBV patients （χ^2 = 13.545, P = 0.000）; the frequency of CC and AC genotype at IL-10 gene promoter -592 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients（χ^2 = 15.970, P=0.000） （χ^2 =20.414, P=0.000）, asymptomatic HBV carriers （χ^2 =21.283, P= 0.000） （χ^2 = 28.309, P = 0.000） and self-restricted HBV patients（χ^2 = 17.047, P = 0.000） （χ^2 = 16.528, P = 0.000） ; the frequency of TC genotype at IL-10 gene promoter -819 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients（χ^2 = 58.961, P = 0.000）, asymptomatic HBV carriers （ χ^2 = 53. 255, P = 0. 001 ） and self-restricted HBV patients （χ^2 = 39.616, P = 0.001）. So interleukine-10 gene polymorphism was associated with the chronic severe hepatitis.The aim of this study is to investigate whether three mononucleotide polymorphisms at the locus -1082,-819 and -592 in the promoter region of the IL-10 gene are associated with chronic severe hepatitis.The IL-10-592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reaction- restriction fragment length polymorphism analysis(PCR-RFLP)while polymerase chain reaction-se- quence specific primer(PCR-SSP)assay was used to test the IL-10-819 polymorphism.The polymor- phisms of IL-10-1082,-819 and -592 genes were detected in 98 patients with chronic severe hepatitis (CSH),478 patients with chronic hepatitis B(CHB),223 asymptomatic(chronic)HBV carriers (ASC)and 267 patients with self-restricted HBV.There was significant difference of the polymor- phisms of IL-10-1082,IL-10-819 and IL-10-592 genes between CSH group and other groups.The fre- quency of AA genotype at IL-10 gene promoter -1082 locus in chronic severe hepatitis patients was higher than that in asymptomatic HBV carriers(X^2=13.314,P=0.001),and self-restricted HBV patients(X^2=13.545,P=0.000);the frequency of CC and AC genotype at IL-10 gene promoter -592 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients(X^2= 15.970,P=0.000)(X^2=20.414,P=0.000),asymptomatic HBV carriers(X^2=21.283,P= 0.000)(X^2=28.309,P=0.000)and self-restricted HBV patients(X^2=17.047,P=0.000)(X^2 =16.528,P=0.000);the frequency of TC genotype at IL-10 gene promoter-819 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients(X^2=58.961,P=0.000), asymptomatic HBV carriers(X^2=53.255,P=0.001)and self-restricted HBV patients(X^2= 39.616,P=0.001).So interleukine-10 gene polymorphism was associated with the chronic severe heoatitis.

关 键 词：Chronic severe hepatitis Interleukin-10 Genotype 

分 类 号：R512.6[医药卫生—内科学]