Phylogenetic analysis of the genomes of two strains of human adenovirus type 3  

作　　者：RONG ZHOU XIAO BO SU QI WEI ZHANG QI YI ZENG BING ZHU CHU YU ZHANG HOE BO WU ZAO HE WU SI TANG GONG 

机构地区：[1]Central Laboratory, Guangzhou Children＇s Hospital, Guangzhou , P . R . China [2]South China Sea Institute of Oceanology, LED, the Chinese Academy of Sciences, Guangzhou, P. R.China [3]State Key Laboratory of Virology, Wuhan University, Wuhan, P. R. China [4]Graduate School of the Chinese Academy of Sciences, Beijing, P. R. China

出　　处：《Journal of Microbiology and Immunology》2007年第2期139-143,共5页中华微生物学和免疫学（英文版）

摘　　要：Human adenovirus type 3 （HAdV-3） is widely prevalent all over the world, especially in Asia. The objective of this study is to carry out complete genomic DNA sequencing and the phylogenetic analysis for two strains （Guangzhou01 and Guangzhou02） of HAdV-3 wild virus isolated from South China. Nasopharyngeal secretion aspirate specimens of sick children were inoculated into HEp-2 and HeLa culture tubes, and the cultures were identified by neutralization assay with type-specific reference rabbit antiserum. Type-specific primers were also utilized to confirm the serotype. The restriction fragments of HAdV genome DNA were cloned into pBlueScript SK （ ＋ ） vectors and sequenced, and the 5＇ and 3＇ ends of the linear HAdV-3 genome were directly sequenced with double purified genomic DNA as templates. General features of the HAdV-3 genome sequences were explored by using several bio-software. Phylogenetic analysis was done with MEGA 3.0 software. The genomic sequences of Guangzhou01 and Guangzhou02 possess the same 4 early regions and 5 late regions and have 39 coding sequences and two RNA coding sequences. Other non-coding regions are conservative. Inverted repeats and palindromes were identified in the genome sequences. The genomes of group B human adenovirus as well as HAdV-3 have close phylogenetic relationship with that of chimpanzee adenovirus type 21. The genomic lengths of these two isolated strains are 35 273 bp and 35 269 bp, respectively. The phylogenetic analysis showed that HAdV-B species has some relationship with certain types of chimpanzee adenovirus.Human adenovirus type 3(HAdV-3)is widely prevalent all over the world,especially in Asia.The objective of this study is to carry out complete genomie DNA sequencing and the phylogenetic analysis for two strains(Guangzhou01 and Guangzhou02)of HAdV-3 wild virus isolated from South Chi- na.Nasopharyngeal secretion aspirate specimens of sick children were inoculated into HEp-2 and HeLa culture tubes,and the cultures were identified by neutralization assay with type-specific reference rabbit antiserum.Type-specific primers were also utilized to confirm the serotype.The restriction fragments of HAdV genome DNA were cloned into pBlueScript SK(+)vectors and sequenced,and the 5' and 3' ends of the linear HAdV-3 genome were directly sequenced with double purified genomic DNA as tem- plates.General features of the HAdV-3 genome sequences were explored by using several bio-software. Phylogenetic analysis was done with MEGA 3.0 software.The genomic sequences of Guangzhou01 and Guangzhou02 possess the same 4 early regions and 5 late regions and have 39 coding sequences and two RNA coding sequences.Other non-coding regions are conservative.Inverted repeats and palindromes were identified in the genome sequences.The genomes of group B human adenovirus as well as HAdV-3 have close phylogenetic relationship with that of chimpanzee adenovirus type 21.The genomic lengths of these two isolated strains are 35 273 bp and 35 269 bp,respectively.The phylogenetic analysis showed that HAdV-B species has some relationship with certain types of chimpanzee adenovirus.

关 键 词：Human adenovirus type 3 Genome Phylogenetic analysis 

分 类 号：R37[医药卫生—病原生物学]