纳米羟基磷灰石沉积层/钛酸钾薄层/钛合金生物复合材料体外培养成骨细胞的研究  

In vitro co-culture of osteoblasts with hydroxyapatite/K_2Ti_6O_(13)/beta-titanium bio-composites

作　　者：李建光[1] 崔春翔[2] 戚玉敏[2] 刘双进[2] 邓嘉胤[3] 彭诚[3] 

机构地区：[1]天津医院脊柱外科,天津市300211 [2]河北工业大学材料学院,天津市300130 [3]天津医科大学口腔医院,天津市300070

出　　处：《中国组织工程研究与临床康复》2007年第35期6924-6928,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(50271024);河北省自然科学基金资助项目(E2006000025)~~

摘　　要：在制备出具有表面活性的纳米羟基磷灰石沉积层/钛酸钾薄层/钛合金(HA/K2Ti6O13/β-Ti)生物复合材料的基础上,将体外培养的成骨细胞与HA/K2Ti6O13/β-Ti生物复合材料、未经处理β钛合金两种骨替代材料共同培养,在既定时间内观察两种骨替代材料对成骨细胞生长、附着的影响。结果表明两种骨替代材料对成骨细胞生长无明显抑制或促进作用,均具有良好的细胞相容性,它们皆能使成骨细胞附着于各自材料表面,分泌形成胶原纤维样基质。HA/K2Ti6O13/β-Ti生物复合材料较β钛合金具有更优异的的生物活性和成骨性能,是一种很好的生物植入材料。Based on the hydroxyapatite （HA）/K2Ti6O13/β-Ti bio-composite with a good bio-activity, the in vitro cultured osteoblast cells were co-cultured on the surface of the substitutes for bone tissues, including HA/K2Ti6O13/β-Ti bio-composite and β-titanium alloys. The proliferation and attachment of osteoblast cells were observed within certain time. The results showed that neither HA/K2Ti6O13/β-Ti nor titanium alloys had an obvious effect on inhibiting or promoting the proliferation of the cells. Both materials were biocompatible to osteoblast cells and promoted the attachment of osteoblasts to material Surface, then the cells excreted the collagen fibril-like matrix on the surface of both materials. It is indicated that HA/K2Ti6O13/β-Ti bio-composite with a more excellent bioactivity and a bone-forming property is a better bio-implant for bone tissues.

关 键 词：成骨细胞 骨替代材料 羟基磷灰石 钛合金 生物复合材料 

分 类 号：R318.08[医药卫生—生物医学工程]