硼硅酸盐生物玻璃的生物活性测试及其细胞相容性  

Bioactivity and cytocompatibility of borosilicate bioglass

作　　者：宁佳[1] 姚爱华[1] 付海罗[1] Qiang Fu1 王德平[1] 黄文旵[1] M N Rahaman 刘欣[1] 

机构地区：[1]同济大学材料学院生物与信息材料研究所,上海市200092 [2]密苏里-罗拉大学生物材料研究中心,罗拉市,密苏里州,美国MO 65409

出　　处：《中国组织工程研究与临床康复》2007年第35期7041-7043,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：上海市科委基础重大项目资助(05DJ14006)~~

摘　　要：目的:测定硼硅酸盐玻璃的生物活性及其细胞相容性,开发一类以硼作为玻璃结构网络主要形成体的生物活性玻璃。方法:实验于2005-10/2006-08在上海同济大学材料科学与工程学院实验室完成。在硅酸盐生物玻璃,45S5玻璃,中引入B2O3,制备不同硼含量(摩尔百分比为15.4,30.7,46.1)的用于骨组织工程材料硼硅酸盐玻璃。以45S5玻璃为对照,通过X射线衍射方法确定生成羟基磷灰石晶体的速度,来评价硼硅酸盐的生物活性;在美国密苏里-罗拉大学生物实验室作成骨细胞在玻璃片上的接触实验和黏附试验,并通过扫描电镜和光学显微镜评价硼硅酸盐玻璃对成骨细胞的相容性。结果:①生物活性:随着硼含量提高,硼硅酸盐玻璃的羟基磷灰石生成速度加快,结晶度增加,说明它们的生物活性逐渐提高。②细胞相容性:与45S5玻璃相比,硼含量高的玻璃,对成骨细胞生长有抑制作用;但在玻璃片界面附近或玻璃片表面的成骨细胞,总体上仍然有较高的存活率,呈现出良好的生长形态。结论:硼硅酸盐玻璃是一类很有发展前景的骨组织工程支架材料,它不仅具有良好的生物活性,而且对成骨细胞具有较好的细胞相容性。AIM： To determinate the bioactivity and cytocompatibility of borosilicate bioglass, and develop a new kind of bioglasses which mainly contain boron as glass network former in the structure. METHODS： The experiment was carded out in the laboratory of School of Materials Science and Engineering of Tongji University from October 2005 to August 2006. By introducing B203 into silicate glass （45S5 glass）, the borosilicate bioglasses used for bone tissue engineering scaffolds, were prepared at different boron contents （15.4, 30.7, 46.1 mol%）. The bioactivity of these borosilicate glasses was evaluated by the formation rate of hydroxyapatite （HAp） crystals with X-ray diffraction method. Taking 45S5 glass as a control, the cytocompatibility to osteoblast cells of these borosilicate glasses was assessed by contact and attachment tests using optical microscope and scanning electron microscope, which were performed at the Biomaterials Research Group at the University of Missouri-Rolla. RESULTS： ① Bioactivity： The formation rate of HAp on the samples increased with the increasing of boron content, so did the crystallinity of HAp. The bioactivity improved with increasing of boron content.② Cytocompatibility： Comparing with 45S5 bioglass, the borosilicate glass with high content boron did have certain inhibition on the growth of osteoblast cells, but osteoblast cells which attached on the borosilicate glass plate surface or the ones near the interface of glass plate still presented good growth on the whole. CONCLUSION： Borosilicate bioglasses may serve as a promising scaffold matedal for bone tissue engineering. They not only have great bioactivity, but also have a good cytocompatibility to osteoblast cells.

关 键 词：硼硅酸盐玻璃 生物活性 细胞相容性 生物材料 

分 类 号：R318[医药卫生—生物医学工程]