人类白细胞抗原G表达与不明原因反复自然流产关系的探讨  

Relationship between human leukocyte antigen-G expression and recurrent spontaneous abortion

作　　者：孙晓光[1] 刘欣燕[1] 杨玉雯[1] 刘彤[2] 

机构地区：[1]中国医学科学院北京协和医院妇产科,100032 [2]中国医学科学院北京协和医院病理科,100032

出　　处：《北京医学》2007年第9期513-516,共4页Beijing Medical Journal

基　　金：首都医学发展科研基金资助项目(2002-3027)

摘　　要：目的通过观察人类白细胞抗原G(HLA-G)在不同滋养细胞中的表达,探讨其与不明原因反复自然流产(RSA)的关系及免疫调节机制。方法用免疫酶细胞化学染色方法检测11例RSA患者滋养细胞的HLA-G表达情况,并以13例正常早孕的滋养细胞和JEG-3绒癌细胞作对照。结果RSA患者滋养细胞的HLA-G表达水平(1.15±1.56)显著低于正常早孕滋养细胞(8.97±2.76)和JEG-3绒癌细胞(9.56±1.81)(P﹤0.001)。后两种滋养细胞的HLA-G表达水平无显著性差异。RSA组5例表达为弱阳性,6例为阴性。正常早孕组8例表达为强阳性,5例为中等阳性。JEG-3绒癌细胞株表达为强阳性。结论滋养细胞的HLA-G表达与细胞浸润能力有关。RSA滋养细胞的低浸润能力可能与HLA-G的低表达有关。HLA-G表达可能不依赖母体环境,而在细胞内自身调节。To investigate the expression of human leukocyte antigen-G （HLA-G） in different trophoblast cells, and to explore how the expression of HLA-G may be related to mechanism of recurrent spontaneous abortion （RSA）. Methods The expression of HLA-G protein was tested in isolated trophoblast cells of 11 cases of RSA by immunoperoxidase cell stain. Normal trophoblast cells from 13 cases of first trimester normal pregnancy, and JEG-3 choriocarcinoma cells were used as controls. Results Immunocytochemistry analysis showed that the expression level of HLA-G in trophoblast cells in RSA group was significantly lower than that of normal pregnant group and JEG-3 cells（P 〈 0.001）, while the difference of HLA-G expression level between the later groups was not significant （P 〉 0.05）. Five cases had very weak HLA-G expression and 6 cases had no HLA-G expression in the RSA group; In the normal pregnant group, 8 had strong positive HLA-G expression, 5 had positive expression. JEG-3 choriocarcinoma cells showed strong HLA-G expression. Conclusions HLA-G expression in trophoblast cells is correlated to the capacity of cell invasion; A low expression of HLA-G may be responsible for the decreased capacity of cell invasion in RSA; The HLA-G expression is regulated in an autocrine manner independent of maternal environment.

关 键 词：人类白细胞抗原G 反复自然流产 滋养细胞 

分 类 号：R714.21[医药卫生—妇产科学]