猪a1-岩藻糖转移酶基因(FUT1)M857位点遗传变异分析  

Analysis of genetic variations at M857 locus of the a1-Fucosy-transferase (FUT1) ORF in pigs

作　　者：吴圣龙[1] 包文斌[1] 鞠慧萍[1] 朱国强[2] 李碧春[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]扬州大学兽医学院,扬州225009

出　　处：《遗传》2007年第9期1071-1076,共6页Hereditas（Beijing)

基　　金：江苏省高技术研究计划项目(编号:BG2006302);扬州大学自然科学基金资助项目(编号:NK0513119)~~

摘　　要：肠毒素大肠杆菌(ETEC)F18是引起仔猪断奶后水肿和腹泻病的主要病原菌,a-1岩藻糖转移酶(FUT1)基因是ECECF18侵染猪小肠的受体蛋白基因。利用PCR-RFLP方法检测了1个野猪以及20个中外家猪猪种(群)共696个个体在FUT1基因开放阅读框架的857核苷酸位点的遗传变异,结果表明:在所有猪种中,均未检测到抗性的AA型纯合子,在外来猪种杜洛克和约克夏、国内猪种临高猪和杂交猪种中检测到AG型杂合子,外来猪种中的皮特兰、长白猪以及除临高猪外的所有国内猪种和野猪均表现为极端的单态分布,只有易感的GG基因型。研究结果提示,中国地方猪种不具备抵抗ETECF18大肠杆菌的遗传基础,与外来猪种确实存在差异,这种差异可能与各自不同的起源有关,ETECF18抗性基因可能起源于欧洲野猪;并推测猪种的生长速度与ETECF18大肠杆菌病的发生具有密切的关系。Enterotoxigenic Escherichia coli FI8 （ETEC FI8） is the main pathogen that causes edema disease and post-weaning diarrhea in piglets, and al-fucosytransferase （FUT1） gene has been identified as a receptor gene encoding the receptor for ETEC F18 bacteria. In this study, the method of PCR-RFLP was used to investigate the among 21 breeds including one wild boar breed and 20 western commercial and Chinese native pig breeds （populations）. The results showed that none of the individuals in all 21 breeds possessed the resistant AA genotype, the genetic polymorphisms of the FUT1 locus were only detected in two western pig breeds （Duroc and Yorkshire）, Lingao pig and hybrid pig breeds, while the wild boar and all the other Chinese pig breeds only possessed the susceptible GG genotype. The results indicated that Chinese native pig breeds, unlike western pig breeds, lack the genetic background on the resistance to ETEC F18 bacteria. This may be owe to their different origination, as the resistance gene to ETEC F18 might be originated from European wild boar. It was also inferred that edema disease and post-weaning diarrhea caused by ETEC F18 had close relationship with the growth speed of pigs.

关 键 词：大肠杆菌F18 FUT1基因 遗传变异 

分 类 号：S828[农业科学—畜牧学]