山羊MyoD基因家族多态性及与体尺性状的相关性  

Association of variations in MyoD family of genes with body measurement traits in goat

作　　者：张海军[1] 陈宏[1] 房兴堂[1] 张润锋[2] 鲍斌[1] 高雪原[1] 邵汝英[1] 

机构地区：[1]徐州师范大学细胞与分子生物学研究所 [2]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《遗传》2007年第9期1077-1082,共6页Hereditas（Beijing)

基　　金：徐州师范大学研究生科研课题(编号:06YL0013);徐州师范大学重点人才基金(编号:XY200234);西北农林科技大学拔尖人才支持计划项目资助~~

摘　　要：用PCR-SSCP技术研究了波尔山羊和徐淮山羊2个群体共147个个体MyoD基因家族中3个基因座位的单核苷酸多态性(single nucleotide polymorphism,SNP)。结果表明,在徐淮白山羊群体中,myf-5基因座发现有3种基因型AA、AB和BB,波尔山羊均为AA型。在myf-6基因座和myoD5′侧翼区基因座,两个山羊群体均检测到了AA和AB型个体。对山羊myf-5、myf-6基因座,myoD5′侧翼区基因座不同基因型与两品种山羊体尺性状相关分析表明,myf-5基因座对管围和管围指数的效应差异显著(P<0.05)。myf-6基因座对徐淮白山羊体尺性状的效应均不显著(P>0.05),而对波尔山羊的体高和管围指数效应差异显著(P<0.05)。两个山羊品种myoD5′侧翼区不同基因型个体的体尺性状差异均不显著。Single nucleotide polymorphisms （SNPs） of hircine myf-5, myf-6 genes and 5＇flanking region of the myoD gene （myoD 5＇） were analyzed by PCR-SSCP in Boer goat and Xuhuai white goat. Three genotypes were found at the myf-5 locus of Xuhuai white goat, denoted AA, AB and BB, while all of Boer goats were ofAA genotype, Only AA and AB genotypes were found at myf-6 and myoD 5＇ loci. The effects of genotypes of myf-5, myf-6 and myoD 5＇flanking region loci on body measurement traits were estimated. The results showed that genotypes at the myf-5 locus were significantly associated with hucklebone width and hucklebone width index in Xuhuai white goat （P〈0.05）. Genotypes at the myf-6 locus were significantly associated with withers heights and hucklebone width index in Boer goat （P〈0.05）, but no significant associations were found for any traits in Xuhuai white goat. The myoD 5＇ locus was not significantly associated with any traits in both types of goat.

关 键 词：山羊 myf-5 myf-6 MYOD 遗传多态性 

分 类 号：S827[农业科学—畜牧学]