家蚕MLP基因的克隆及其结构分析  

Cloning and structural analysis of MLP in the silkworm,Bombyx mori

作　　者：刘岩[1] 牛宝龙[1] 翁宏飚[1] 沈卫锋[1] 何丽华[1] 齐晓朋[1] 孟智启[1] 

机构地区：[1]浙江省农业科学院蚕桑研究所,杭州310021

出　　处：《遗传》2007年第9期1097-1102,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30371087);国家高技术研究发展计划(863计划)项目资助(编号:2006AA10A119)~~

摘　　要：利用生物信息学的方法快速获得家蚕MLP(Muscle LIM protein,MLP)基因cDNA电子序列,经RT-PCR生物验证正确,登录GenBank(No.DQ311195)。MLP基因cDNA长2327bp,ORF全长1485bp,编码产生494个氨基酸。该MLP基因组DNA含有11个外显子,10个内含子,所有内含子/外显子边界都符合典型的GT/AG剪切模式。MLP基因编码的蛋白富含Gly(14.4%),分子量约为53.03kDa,等电点(PI)为8.29。通过BLAST分析发现该基因编码的家蚕肌肉LIM蛋白,含有5个保守的LIM结构域,家蚕的另一种LIM蛋白(AAR23823)含一个LIM结构域,两者可能是通过可变剪切产生;后者可能通过竞争作用调节前者在肌细胞中的功能。MLP的克隆为进一步研究其体内功能奠定了基础。The LIM domain is found in a wide variety of eukaryotic proteins that regulate gene expression and cell differentiation during development. Muscle LIM protein （MLP） gene in Bombyx mori has been cloned by blasting its EST database and PCR test in present report. The resulting sequence covers 2 327 bp of cDNA （GenBank accession No. DQ311195）. It has a complete open reading fragment and encodes a 494 amino acid protein. Genomic DNA sequence contains 11 exons and l0 introns, with intron splicing following the GT-AG rule. M.W. and PI of the predicted MLP in Bombyx mori are 53.03 kDa and 8.29 respectively. A single LIM domain linked to a glyscine-rich region is found in a previously deposited LIM protein （AAR23823） in Bombyx mori. MLP identified in this report encodes a protein with five tandem LIM-glycine modules. The two LIM proteins could be produced by alternative splicing and both are probably involved in muscle cell differentiation. This work provides foundation for further research on the in vivo function of MLP.

关 键 词：家蚕 MLP 表达序列标签(EST) LIM结构域 

分 类 号：S881.26[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]