45S rDNA在小麦及其近缘物种染色体上的分布  

Distribution of 45S rDNA sequence on chromosomes of Triticum aestivum and its relative species

作　　者：徐川梅[1] 别同德[1] 王春梅[1] 周波[1] 陈佩度[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室

出　　处：《遗传》2007年第9期1126-1130,共5页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30270827)~~

摘　　要：将染色体C-分带和原位杂交技术相结合,系统研究了45S rDNA在栽培一粒小麦、野生二粒小麦、普通小麦、大麦、簇毛麦、硬簇麦、六倍体燕麦及鹅观草等物种染色体上的分布情况。这些物种染色体的次缢痕区都有45S rDNA位点,某些非随体染色体上也有45S rDNA位点分布。以小麦—鹅观草1Rk#1二体附加系为材料,通过顺序C分带-FISH技术首次将一个45S rDNA定位到1Rk#1染色体短臂末端。Fluorescent in situ hybridization （FISH） and sequential C-banding and FISH techniques were used in locating 45S rDNA sequence on specific chromosomes of Triticum monococcum, T. dicoccoides, T. aestivum, Hordeum vulgare, Haynaldia villosa, T. durum-Haynaldia villosa amphiploid, Avena fatus, and Roegneria kamoji. High polymorphism between common wheat and its relatives on 45S rDNA sequence distribution was detected. All the chromosomal secondary constriction regions of these species had 45S rDNA loci. Some non-satellite chromosomes had additional 45S rDNA loci. A 45S rDNA locus was located on the terminal region of the short arm of R. kamofi chromosome 1Rk^#1.

关 键 词：45S RDNA 顺序C分带-FISH技术 小麦近缘物种 

分 类 号：S512.1[农业科学—作物学] Q943[生物学—植物学]