不同丙酸/乙酸对聚糖菌长/短期代谢的影响  

Long-Term and Short-Term Effects of Propionic/Acetic Acid Ratios on Metabolism of Glycogen-Accumulating Organisms

作　　者：姚樱[1] 陈银广[1] 马民[1] 顾国维[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092

出　　处：《环境科学》2007年第9期1970-1974,共5页Environmental Science

基　　金：国家自然科学基金项目(50408039);国家高技术研究发展计划(863)项目(2004AA649330);霍英东教育基金会资助项目(101080)

摘　　要：在3个SBR反应器(即SBR-A,SBR-C和SBR-E)中,以3种不同比例的丙酸/乙酸中长期驯化活性污泥使之富集聚糖菌,对丙酸/乙酸对聚糖菌的长期和短期代谢的影响进行了研究.结果表明,长期高丙酸/乙酸驯化导致聚糖菌厌氧消耗的糖原量和合成的聚β羟基烷酸酯(poly--βhydroxyalkanoates,PHA)的量都较少,在好氧段积累的糖原量和降解的PHA的量也较小,并且对聚糖菌的脱氢酶活性、生长速率及对有机酸的利用能力等都有抑制作用.当进水中的丙酸与乙酸的碳摩尔数相同时,单位生物量利用乙酸的速率较丙酸快.不同丙酸/乙酸对聚糖菌代谢的短期影响的研究表明,当进水中乙酸浓度突然提高时,长期以高丙酸/乙酸驯化的聚糖菌可以直接利用乙酸参与代谢,但长期以低丙酸/乙酸驯化的聚糖菌则无法快速利用丙酸.提高进水中丙酸/乙酸有可能成为有效抑制聚糖菌生长并提高增强生物除磷系统稳定性的一种策略.Three activated sludges enriched with glycogen accumulating organisms （GAO） were acclimatized respectively with different ratios of propionic to acetic acid（i.e.biomass SBR-A,C and E）.The effect of different ratios of propionic/acetic acid on the metabolism of long-term cultivated GAO was investigated. Cultivated with high propionic/acetic acid ratio,GAO consumed less glycogen and synthesized less poly-β-hydroxyalkanoates（PHA） in the anaerobic phase,and in the aerobic phase accumulated less glycogen and degraded less PHA,and at the same time the microbial growth was lower.When the carbon mole of acetic acid equaled that of propionic acid in the influent,GAO utilized acetic acid faster than propionic acid.Batch tests were carried out with biomass SBR-A and SBR-E to study the transient response of long-term cultivated GAO to short-term change of propionic/acetic acid ratio.The GAO cultivated with a high propionic/acetic acid ratio was able to utilize acetic acid immediately when the concentration of aceticacid in the feed suddenly increased.But when the biomass cultivated with a low propionic/acetic acid ratio was feed with high ratio propionic/acetic acid wastewater,the propionic acid uptake rate was only 41.1% of the rate of the GAO long-term cultivated with high propionic/acetic acid.The sudden increase of propionic/acetic acid ratio could effectively inhibit the metabolism of GAO.

关 键 词：聚糖菌 乙酸 丙酸 增强生物除磷 

分 类 号：X703.1[环境科学与工程—环境工程]