参附注射液对急性坏死性胰腺炎肺损伤保护作用的研究  

PROTECTIVE STUDY OF SHENFU INJECTION ON LUNG INJURY FOLLOWING ACUTE NECROSIS PANCREATITIS IN RATS

作　　者：罗怡君[1] 刘欣[2] 李朝阳[1] 黄宝胜[1] 吴冬[1] 

机构地区：[1]广东医学院附属深圳南山医院,广东深圳518052 [2]广东医学院麻醉教研室,广东湛江524023

出　　处：《现代医院》2007年第9期33-34,共2页Modern Hospitals

摘　　要：目的探讨急性胰腺炎时,血清TNF-α、IL-10水平和肺组织MDA含量、SOD活性的变化及参附注射液对它们的影响。方法健康SD大鼠30只分成3组:手术对照组(S组)、急性坏死性胰腺炎组(ANP组)、参附组(SF组),每组10只。采用5%牛磺胆酸钠诱导制备大鼠急性坏死性胰腺炎(ANP)模型。S组和ANP组于术前30分钟开始分别静脉泵注生理盐水10ml.kg-1.h-1,而SF组于术前30分钟开始静脉泵注参附注射液10mg·kg-1.h-1(1mg稀释为1ml)。4小时后活杀,每只大鼠取一叶左肺,光镜观察组织学改变。采用ELISA检测大鼠血清的TNF-α与IL-10水平;硫代巴比妥酸比色法测定丙二醛(MDA)含量;黄嘌呤氧化酶法测定过氧化物歧化酶(SOD)活性。结果MDA含量和TNF-α水平ANP组显著高于S组和SF组(p<0.01),且SF组MDA含量高于S组(p<0.05);S组和SF组SOD活性高于ANP组(p<0.05);IL-10水平ANP组显著低于S组(p<0.01),且低于SF组(p<0.05)。结论参附注射液能降低肺组织MDA含量和血清TNF-α水平,升高IL-10水平,保护SOD活性,对急性坏死性胰腺炎肺损伤有保护作用。Objective To study the protective effect of Shenfu injection on llLung injury following acute necrosis pancreatitis. Methods 30 SD rats were randomized into three groups each consisting of 10 rats ： Sham - operated control group （S group）, acute necrosis pancreatitis group （ANP group）, Shenfu group （SF group）, ANP models were induced byretrograde injection of 5% sodium taurocholate in the pancreatic duct. TNF- α, IL- 6 were detected by ELISA method. MDA was measured by thiobarbiturate colorimetry, and SOD activity was detected by xanthine oxidase technique. Results The content of MDA, TNF - α in ANP group was significandy higher than those in S and SFgroup （p 〈0. 01 ） ,and MDA in SF group was higher than in S group （p 〈0.05）. The SOD activity in S group and SF group were higher than that in ANP group（p 〈0. 05）. Meanwhile, IL - 10 level in ANP group was significantly lower than in S group （p 〈0. 01 ） ,and that was lower than in SF group （p 〈0. 05 ）. Conclusion Shenfu can decrease MDA content in lung tissue and TNF - α level in blood serum,increase IL - 10 level in blood serum, protect SOD activity. Therefore,it can protect lung injury following acute necrosis pancreatitis.

关 键 词：参附 胰腺炎 肺损伤 

分 类 号：R657.51[医药卫生—外科学]