扶正抑瘤颗粒对H_(22)荷瘤小鼠肿瘤细胞P^(53)、BCL-2蛋白表达的影响  

EFFECTS OF FUZHEN YILIU GRANULE ON P^(53) ANDBCL-2 GENES PROTEIN EXPRESSION IN TUMOR CELLS OF H_(22) SOLID TUMOR MICE

作　　者：沈世林[1] 赵健雄[2] 朱玉真[2] 王学习[2] 徐静汶[2] 王敏[2] 

机构地区：[1]兰州军区兰州总医院 [2]兰州大学,兰州730000

出　　处：《中国中医药科技》2007年第5期316-317,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：甘肃省科技厅攻关项目NoGS012-A34-109;教育部重点项目No01128

摘　　要：目的:观察扶正抑瘤颗粒(FYK)对小鼠肿瘤细胞凋亡相关基因P53、BCL-2蛋白表达的影响。方法:将48只小鼠分成FYK大剂量组、FYK小剂量组、天仙胶囊阳性对照组和荷瘤模型组,造模形成实体瘤后分别灌胃FYK、天仙胶囊、生理盐水18天,取瘤组织用流式细胞仪检测P53、BCL-2蛋白表达。结果:FYK组肿瘤细胞P53蛋白表达升高、BCL-2蛋白表达下降,与模型组相比较有显著性差异(P<0.01)。结论:FYK具有促进肿瘤细胞P53表达和抑制BCL-2表达作用。Objective:To observe the effects of fuzhen yiliu granule on the P53 and BCL-2genes protein expression related to apoptosis in tumor cells of H22 solid tumor mice.Methods:48mice were divided into 4 groups:model group,control group,low and high dose groups,all mice were inoculated H22 tumor strain to build models,4 groups were respectively given NS,tianxian capsule,low and high dose fuzhen yiliu granule for 18 days.P53 and BCL-2 genes protein expression were determined by flow cytometry technique.Results:The P53 genes protein expression was lower and the BCL-2 expression was higher obviously in model group,the P53 expression was elevated and BCL-2 expression was lowered in fuzhen yiliu granule groups,there was significant difference between fuzhen yiliu granule groups and model group(P<0.01).Conclusion:Fuzhen yiliu granule can promote the P53 expression and inhibit the BCL-2 expression in tumor cells,which maybe one of the mechanism that fuzhen yiliu granule can effect on tumor.

关 键 词：肿瘤/中医药疗法 @扶正抑瘤颗粒/治疗应用 基因表达 小鼠 

分 类 号：R273[医药卫生—中西医结合]