水稻基因表达谱分析寡核苷酸芯片制备的研究  

Establishment of rice oligonucleotide microarray in rice inflorescence development

作　　者：李援亚[1] 曾黎琼[2] 王力[3] 张云孙[4] 

机构地区：[1]云南大学生命科学学院,云南昆明650091 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650223 [3]成都军区昆明总医院,云南昆明650032 [4]云南大学科学技术处,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2007年第5期515-518,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省自然科学基金(青年项目)资助项目(2005C0006Q;2003C0079M)

摘　　要：首先有目的地搜集了50个水稻花序相关基因,进行了探针的设计、筛选及合成纯化,用点样仪以微阵列的形式将其点于醛基化的玻璃片上;将3个不同生长阶段的水稻花序材料的总RNA经荧光标记反转录后与寡核苷酸芯片进行杂交.用ScanArray3000对获得的表达谱进行扫描分析显示,芯片图像背景均匀,信号清晰.用ImaGene4.0软件对表达谱分析表明,候选基因在水稻花序3个不同发育阶段的材料中,表达水平有显著差异.为进一步进行水稻寡核苷酸芯片的制备及应用奠定了基础.Fifty rice inflorescence related genes from Internet,references were chosen. After probes of the 50 genes were designed and screened by BLAST analysis in the Internet, they were synthesized, purified and micro-arrayed onto the microscope sliders. Total RNA from 3 kinds of rice inflorescence tissues was extracted and labeled by fluorescent dye. The labeled probes were then hybridized with the oligonucleotide arrays. Expression patterns of 50 genes from rice inflorescence in different development were obtained by detection using ScanArray3000. It showed analyzed pots with uniformity background and clear signal. The plots of the images were acquired after the acquired gene expression patterns were analyzed by ImaGene4.0 software. The scatter plots and scale maps show that there existed a significant difference in the expression of these candidate genes in rice inflorescences with different development phase. Then,a foundation for the preparation of rice oligonucleotide microarray and farther application was layed.

关 键 词：寡核苷酸芯片 水稻花序相关基因 基因表达谱 

分 类 号：Q949.91[生物学—植物学]