4个微卫星标记对3个绵羊品种肉用性状多态性的研究  

Sheep meat property analysis by using polymorphism of 4 microsatellites DNA in 3 sheep breeds

作　　者：柳楠[1] 孔繁臻[1] 张兴国[1] 石国庆[2] 任航行[2] 代蓉[2] 刘国庆[3] 

机构地区：[1]莱阳农学院动物科技学院,山东青岛266000 [2]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [3]合肥工业大学生物与食品工程学院,安徽合肥230036

出　　处：《甘肃农业大学学报》2007年第4期24-28,共5页Journal of Gansu Agricultural University

基　　金：新疆建设兵团博士基金项目(编号:2003-02);新疆兵团绵羊繁育生物技术重点实验室开放基金(编号:05KLS02)

摘　　要：选用无角陶赛特、萨福克与中国美利奴肉用绵羊161只,经耳组织提取其基因组DNA,并通过4对微卫星引物对其进行PCR扩增,然后通过电泳分型、凝胶成像系统分析各位点等位基因及全部个体的标记基因型,计算基因频率、多态信息含量(PIC)和杂合度等遗传多态性指标.结果表明,BM3413,BP33,MCM38,MCMA26位点的等位基因数分别为12、15、13、13,多态信息含量丰富,杂合度高,均属于高度多态性位点,用于绵羊肉用形状较理想的遗传标记.A group of 161 sheep consisting of Dorset,Suffolk and China Merino for meat were chosen and their population genetic polymorphism was analyzed by the process of genome DNA extraction from ears issue,PCR amplification of 4 primers, fragment typing,allele of every locus and genotype analysis of all individuals by gel imaging system, calculation of allele frequencies polymorphism information content （PIC）and Heterozygosity. The results showed that, the number of alleles in BM3413, BP33, MCM38, MCMA26, was 12,15,13 and 13, and the polymorphism information content（PIC） was abundant, and the heterozygosity was high. All of them were high polymorphic loci and might be ideal loci genetic markers related to the production performance.

关 键 词：微卫星 杂合度 多态信息含量 有效等位基因 

分 类 号：Q343[生物学—遗传学] S826[农业科学—畜牧学]