液相色谱-质谱联用法检测百花定喘丸中掺有的激素成分  

Determination of Hormones in Baihuadingchuan Pill by LC-MS

作　　者：郭瑞锋[1] 郄冰冰[1] 何建峰[2] 

机构地区：[1]河北省药品检验所,河北石家庄050011 [2]河北省邯郸市中医院,河北邯郸056001

出　　处：《中国药业》2007年第18期28-29,共2页China Pharmaceuticals

摘　　要：目的采用两套经过方法学验证的高效液相色谱(HPLC)系统,对百花定喘丸中的激素成分进行分离筛查,并利用液相色谱-质谱联用(LC-MS)对已分离出的激素成分进行确认。方法仪器为岛津LC-MS2010A液相色谱质谱联用仪,质谱分析器为单极四级杆检测器。色谱柱为迪马C18柱,流动相为乙腈-水(梯度洗脱),检测波长200～600nm,流速为1.0mL/min。结果在百花定喘丸中检测出醋酸泼尼松和泼尼松两种激素成分。结论所用方法简便快速,检测结果准确可靠。Objective To establish a LC- MS method for the analysis of hormones in Baihuadingchuan Pill. Methods The hormone was analyzed by Shimadzu LC- MS2010 combined with ES1 mode. The chromatographic column of C18 was used. The mobile phase consisted of acetonitrile - water and the flow rate was 1.0 mL/min. The detection wavelength was 200 - 600 nm. Results There was two kinds of hormone in Baihuadingchuan Pill. Conclusion This method is simple, quick. The result of determination is accurate.

关 键 词：色谱-质谱联用 中药 百花定喘丸 激素 

分 类 号：R927.2[医药卫生—药学] R977.11