临床分离的变形杆菌中整合子分型研究  

Research of classfying integrons in clinical proteus

作　　者：陈晓莉[1] 徐元宏[1] 储洁[1] 黄颖[2] 林祥宏[2] 

机构地区：[1]安徽医科大学临床检验诊断学教研室,安徽合肥230032 [2]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《中国微生态学杂志》2007年第5期408-410,共3页Chinese Journal of Microecology

基　　金：安徽省十五攻关二期科研课题(040130583)

摘　　要：目的了解临床分离变形杆菌中1、2类整合子的流行现状及其耐药性。方法PCR扩增146株变形杆菌中1、2类整合酶基因,M-H肉汤稀释法检测146株变形杆菌的药敏情况。结果1类整合子检出率为36.9%(54/146),2类整合子检出率为38.4%(56/146),同时携带1、2类整合子的检出率为18.5%(27/146)。结论1、2类整合子在变形杆菌感染临床分离株中所占比例已相当高,1、2类整合子与变形杆菌的多重耐药具有相关性。Objective To investigate the distribution of class 1 and class 2 integrons in the clinical proteus and to analyze the correlation between integrons and its resistance. Methods A total of 146 proteus isolates were included in this study. PCR were used for screening of class 1 and class 2 integrons. The M-H broth mierodilution method was used to determine the MIC values of a range of antibiotics for these isolates. Results 54 strains（36.9% ）of 146 clinical isolates were observed in class 1 integron and 56 strains （38.4%） were observed in class 2 integron. 27 of them（ 18.5% ）were showed in class 1 integron and in class 2 integron. Conclusions Class 1,2 integron occurrence rate was quite high in the clinical proteus isolates, and closely associated with the multi-drug resistance of proteus.

关 键 词：变形杆菌 聚合酶链反应 整合子 

分 类 号：R378.2[医药卫生—病原生物学]