负载亚甲基蓝的纳米磁性碳粉修饰电极研制及DNA的测定  

Determination of DNA Using Methylene Blue Modified Magnetic Electrode

作　　者：李建平[1] 费栋[1] 

机构地区：[1]桂林工学院材料与化学工程系,广西桂林541004

出　　处：《传感技术学报》2007年第9期1940-1944,共5页Chinese Journal of Sensors and Actuators

基　　金：教育部科学研究重点项目资助(207087);广西高校百名中青年学科带头人资助计划项目资助(桂教人[2005]64号);广西自然科学基金项目资助(桂科自0679002).

摘　　要：采用化学沉积法在碳粉上负载纳米Fe3O4颗粒,吸附亚甲基蓝后吸着在固体石蜡碳糊电极上,制成了亚甲基蓝修饰的磁性电极.研究了在0.1mol/L的NH4Cl溶液中,DNA对电极上修饰物亚甲基蓝的电氧化还原作用,考察了多种实验条件对峰电流的影响.在-0.6～0.3V电压下,在0～12mg/L范围内DNA浓度与Δia成反比,从而实现对DNA的测定.研究了亚甲基蓝修饰的磁性电极的电化学行为.该磁性修饰电极集分离、富集和测定于一身,且所得的修饰电极具有灵敏度高、稳定性好、表面易更新等优点.A new type of magnetic nano-Fe3O4 was synthesized on graphite particles. Methylene blue （MB） was absorbed on the surface of the magnetic graphite particles, it could be adhered to carbon paste electrode by magnetic force to fabricate MB modified magnetic electrode. The voltammetric behaviour of MB- DNA in 0. 1 mol/L NH4Cl solution was examined; conditions for the determination of DNA were investigated. Concentration of DNA in the range of 0- 12 mg/L is linear to the degradation of current at-0. 6 V. Influence of H^＋ concentration on the electro-behaviors was studied. It was demonstrated that H^＋ participates the reaction. The sensor exhibited many advantages, such as simple fabrication, fast response, high sensitivity and good reproducibility, and can be renewed easily.

关 键 词：磁性纳米碳粉 亚甲基蓝 DNA 修饰电极 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]