基于纳米二氧化硅掺杂的丝网印刷碳糊电极葡萄糖生物传感器  

Glucose Biosensors Based on Screen-Printed Carbon Paste Electrodes Modified by Nano-SiO_2

作　　者：杨笑鹤[1] 杨昊[1] 潘敏[1] 陈裕泉[1] 

机构地区：[1]浙江大学生物传感器国家专业实验室,杭州310027

出　　处：《传感技术学报》2007年第9期1945-1948,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助项目(30470461);国家863项目延伸资助(2003AA404180)

摘　　要：研究了在丝网印刷碳糊电极上制作并改进生物敏感膜的过程,完成了基于纳米二氧化硅掺杂的用于测量人体血浆中葡萄糖浓度的生物传感器的制作.从酶促动力学原理分析了电极和生物敏感膜在各种实验参数优化前后的特性.实验证明:优化后的传感器灵敏度提高了2.6倍,检测范围为1.1～33.3mmol/L,对测试范围内不同浓度的葡萄糖样本,相对标准偏差均<5(,且稳定性良好,有较高的研究价值和应用前景.The process of manufacturing and improving biologic sensitive membrane was completed, and the biosensors for measuring glucose concentration in blood plasma was completed. According to enzymatic reaction kinetics, both characters of electrodes and biologic sensitive membrane, which were optimized and no optimized, were analyzed. The experiment results showed that the sensitivity of optimized was 2. 6 times of no optimized. Measurable linear range is 1.1-33. 3 mmol/L. Testing of different concentration glucose： RSD〈5%, and stable. So glucose biosensors modified by nano-SiO2 have highly applied value.

关 键 词：生物传感器 纳米颗粒 反应动力学 灵敏度 葡萄糖氧化酶 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]