用PCR检测肉种鸡的鸡胚、雏鸡和子代肉鸡CAV和ALV  

PCR Detection of CAV and ALV in the Embryonic Chickens and Broiler Chicks from AA Broiler Breeders

作　　者：孙晴[1] 王自然[1] 李同树[2] 

机构地区：[1]山东省临沂师范学院农林学院动物医学系,山东临沂276005 [2]山东农业大学动物科技学院,山东泰安271018

出　　处：《江西农业大学学报》2007年第4期603-607,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：山东省财政支持重点农业科技成果推广项目(SDGP-2004-54-0)

摘　　要：为了解CAV和ALV在不同阶段对肉鸡的感染情况,应用PCR方法,对山东省某3个中小型AA肉种鸡场的鸡胚、1日龄雏鸡和子代肉鸡中的CAV和ALV进行检测,试验直接采集样品的不同组织提取DNA,进行PCR扩增及PCR产物的克隆和序列测定。结果显示,被检的3个肉种鸡场及商品肉仔鸡均有这两种病毒的感染,其中CAV的阳性率24.22%;ALV的阳性率17.56%,两者共感染的阳性率8.89%。而且感染鸡各组织病毒含量也有差异,CAV以脾脏最多,ALV以肾脏最多。同时对肝脏进行细菌分离培养,并对40日龄子代商品肉鸡进行ND血凝抑制(HI)抗体效价检测,发现大肠杆菌等细菌感染阳性率较高,ND-HI抗体效价显著偏低。由此可见,鸡胚、雏鸡和子代肉鸡体内存在CAV、ALV的感染以及与细菌性疾病的共感染,造成机体免疫力下降。CAV （Chicken infectious anemia Virus） and ALV （Avian leukosis virus） were detected by PCR from embryonic chickens , one - day chickens and commercial chickens collected from different 3 AA broiler breeding farms of Shandong Province to see infections of CAV and ALV in broilers in different stages. DNAs from different organs were extracted, amplified,and evaluated by PCR,and the cloned plasmid was sequenced. The results showed that CAV and ALV were proved to be positive in the three farms investigated, and the positive ratio of CAV and ALV were 24.22. % and 17.56%. The co - infection ratio of ALV and CLV was 8.89%. The positive ratios differ in different organs, and the highest positive ratios of CAV and ALV were observed in spleen and kidney, respectively. The pathogens from the liver of the broilers were isolated and identified. At the same time,ND -HI test was used to detect the valence of antibody in 40 d commercial chicken, it was showed that the infection ratio of E. coil was the highest , the valence of HI was lower. The above results demonstrated that the infection of CAV and ALV and the co - infection of other bacteria weaken the immunity of embryonic chickens ,ld chickens and commercial chickens.

关 键 词：肉种鸡 鸡传染性贫血病病毒 禽白血病毒 共感染 抗体 

分 类 号：S831.1[农业科学—畜牧学]