六味地黄汤对宫内发育迟缓胎鼠脑发育的影响  

Effect of Liuwei Dihuang decoction on brain development of intrauterine growth retardation rats

作　　者：蔡光先[1] 刘柏炎[1] 陈奕安[1] 

机构地区：[1]湖南中医药大学,长沙410007

出　　处：《中华中医药杂志》2007年第9期642-644,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金项目(No.03JJY3040)

摘　　要：目的:观察补肾方六味地黄汤对宫内发育迟缓胎鼠脑发育的影响,反证中医"肾"与"脑"的关系。方法:采用怀孕小鼠被动吸烟复制宫内发育迟缓模型,动物随机分为3组,分别给予蒸馏水、黄芪和六味地黄汤,于孕19天处死母鼠,计数总胚胎数、活胎数、吸收胎数、死胎数,称量活胎体质量和脑质量,观察胎鼠大脑显微结构和细胞凋亡情况。结果:被动吸烟可使孕鼠死胎数、吸收胎数增加,仔鼠脑发育迟缓,脑内细胞凋亡较正常仔鼠增加;黄芪、六味地黄汤能减少死胎、吸收胎数,增加仔鼠体质量与脑质量,一定程度改善脑发育,减少凋亡细胞数,与模型组差异有显著性(P<0.05),且黄芪与六味地黄汤比较差异有显著性(P<0.05)。结论:六味地黄汤能显著促进宫内发育迟缓仔鼠脑的发育,提示"肾"通于"脑"理论的合理性。Objective： To observe the effect of Liuwei Dihuang decoction on brain development of intrauterine growth retardation rats,and to demonstrate the relationship between brain and kidney in TCM.Methods： Animals were divided into 4 groups at random： normal group,model group,Huangqi（HQ） and Liuwei Dihuang（LD） treated groups.The IUGR model was established by passive smoking.On the 19th day of pregnancy,all rats were killed;the total numbers of embryos,the lively,dead and absorbed embryos were counted.The body and brain weight of lively embryos were scaled respectively,then microstructure and apoptosis in brain were observed.Results： Passive smoking can result in the number of dead and absorbed embryos increases.Compared with normal group,the number of apoptotic cells of model group increased.Compared with model group,in Huangqi and Liuwei Dihuang treated groups,the number of dead and absorbed embryos decreased apparently,body and brain weight increased obviously,the number of apoptotic cells reduced significantly（P〈0.05）.Moreover,there was significantly difference between HQ and LD group（P〈0.05）.Conclusion： Liuwei DiHuang decoction can improve the brain development of IUGR mouse obviously,then proves the theory that ＂kidney communicated brain＂ in TCM is reasonable.

关 键 词：脑 发育 细胞凋亡 六味地黄汤 

分 类 号：R285.5[医药卫生—中药学]