高效液相-柱后衍生化法测定青蒿素含量  

Determination of artemisinin by HPLC with post-column derivatization

作　　者：申薇薇[1] 王锐利[1] 胡爽[1] 李慧[1] 贾佳[1] 张淑秋[1] 

机构地区：[1]山西医科大学药学院临床药学教研室,太原030001

出　　处：《中国药物与临床》2007年第9期657-659,共3页Chinese Remedies & Clinics

基　　金：国家自然科学基金资助项目(30572367)

摘　　要：目的建立测定青蒿素含量的测定方法。方法采用反相高效液相色谱(HPLC)-柱后衍生化-紫外检测法,流动相为乙腈-甲醇-醋酸盐缓冲液(pH4.0)(60∶20∶20),流速0.5ml/min;衍生试剂为1mol/L氢氧化钾(KOH)的90%乙醇溶液,流速0.3ml/min;反应温度:70℃,柱温:30℃,检测波长:289nm。结果青蒿素在50～2000ng/ml范围内线性关系良好,以峰面积对青蒿素浓度进行线性回归,方程为A=91.3C-818.9,r=0.9998;日内、日间精密度相对标准差(RSD)均<2%;平均回收率98%～102%。结论该法准确、简便、重现性好,可用于青蒿素及其制剂或提取物的质量控制。Objective To establish a method for determining artemisinin by HPLC with post-column derivatization and UV detection. Methods HPLC method was used with the mobile phase consisted of acetonitrile, methanol and 0.1 mol/L acetate bufler （pH 4.0） （60：20：20, v/v）, delivered at a flow-rate of 0.5 ml/min. 1 mol/L potassium hydroxide in 90% ethanol as post-column derivatization reagent was delivered at a flow-rate of 0.3 ml/min. The temperature of post-column reaction was set at 70 ℃. Column temperature was kept at 30 ℃, and the working UV wavelength was set to 289 nm. Results The linear range of artemisinin concentrations was 50-2000 ng/ml. The lower limit of quan tification was 50 ng/ml. The inter- and intra-day coefficients of variation were less than 2 %. Mean recovery was 98.0%- 102.0%. Conclusion The method presented in this study showed good accuracy, simplicity and reproducibility as a recommendable option ibr quality control of artemisinin and its preparations or extracts.

关 键 词：青蒿素 色谱 高效液相法 柱后衍生 含量测定 

分 类 号：R284[医药卫生—中药学]