黄波罗的愈伤组织培养  

Callus Culture of Phellodendron amurense Rupr.

作　　者：亓磊[1] 詹亚光[2] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]东北林业大学生命科学学院

出　　处：《东北林业大学学报》2007年第9期14-15,23,共3页Journal of Northeast Forestry University

基　　金：大庆市科学技术计划项目(大庆地区抗旱耐盐绿化树种资源的选择;引种及产业化繁殖技术研究)

摘　　要：通过组织培养技术研究了NAA、NAA与6-BA组合、培养基类型、培养基pH值、蔗糖质量浓度、接种量、培养时间及继代次数对黄波罗愈伤组织生长的影响。结果表明:单独使用NAA时,以1.0mg.L-1愈伤组织生长率最高;NAA与6-BA组合,以0.3mg.L-16-BA+1.0mg.L-1NAA最好,其生长量明显大于单独使用NAA的处理。黄波罗愈伤组织最佳的培养体系为:NT+0.3mg.L-16-BA+1.0mg.L-1NAA,培养基pH值为6.3左右,蔗糖质量浓度为20g.L-1,接种量0.4g,继代时间为20d,愈伤组织生长量在培养4代后达到最大,达2.14g左右。The effects of different concentrations of NAA, combination of NAA and 6-BA, types of culture media, pH of media, carbon sources, inoculating quantity, culture time, and subculture number on callus culture of Phellodendron amurense were studied by tissue culture. Results show that 1.0 mg·L^-1 NAA is the best for callus culture when it is used singly. The optimal combination is 1.0 mg· L^-1 NAA ＋ 0.3 mg· L^-1 6-BA when both NAA and 6-BA are used, and the callus yield is significantly higher than that using NAA singly. The best culture system of P. amurense is obtained as medium supplemented with NT, 0.3 mg· L^-1 6-BA and 1.0 mg· L^-1 NAA, pH 6. 0, carbon sources 20 g· L^-1 , inoculating quantity 0.4 g, and culture time 20 days. The callus yield up to 2.14 g after the subculture is repeated 4 times.

关 键 词：黄波罗 愈伤组织 继代培养 

分 类 号：Q813.1[生物学—生物工程]