心肌营养素-1在阿霉素心衰大鼠心室重构中的作用及伊贝沙坦对其的干预  

Expression of cardiotrophin-1 and effects of irbesartan in adriamycin-induced cardiomyopathy in rats

作　　者：庄汉屏[1] 姚佳梅[1] 陈英[1] 李传昶[1] 

机构地区：[1]中南大学湘雅医院老干科,长沙410008

出　　处：《中南大学学报（医学版）》2007年第4期574-578,共5页Journal of Central South University ：Medical Science

基　　金：湖南省教育厅科学研究重点项目(湘财[2006]36号);湖南省卫生厅资助项目(B2006-038)~~

摘　　要：目的:探讨血管紧张素Ⅱ受体1拮抗剂伊贝沙坦对阿霉素心衰大鼠心室肌中心肌营养素-1(CT-1)的表达以及对心室重构的影响。方法:30只雄性SD大鼠,随机分成2组:正常对照组(n=10)和心衰模型组(n=20)。后者给予腹腔注射阿霉素造模,造模后将存活的18只大鼠随机分为模型对照组和治疗组,分别给予生理盐水(2mL/d,Qd)和伊贝沙坦(50mg/kg,Qd)灌胃。同时正常对照组也给予生理盐水(2mL/d,Qd)灌胃。灌胃12周后用免疫组织化学的方法检测CT-1的表达。结果:与正常对照组相比,模型对照组和治疗组大鼠CT-1的表达均明显升高,模型对照组大鼠心室肌CT-1和左心室质量指数呈明显正相关,治疗组的CT-1较模型对照组明显降低。结论:CT-1的过度激活在阿霉素心力衰竭大鼠的心室重构中发挥着重要作用,伊贝沙坦逆转心室重构的作用可能与其下调CT-1的表达有关。Objective To investigate cardiotrophin-1 （ CT-1 ） expression in the ventricle and the effects of angiotensin Ⅱ type Ⅰ receptor antagonist （ AT1RA ） irbesartan oh the ventricular remodeling in adriamycin myocardiopathy. Methods Thirty male SD rats were randomized into 2 groups： a control group （ n = 10 ） and a model group （ n = 20 ）. The model group was administered adriamycin and 18 rats survived. And theses rats were randomized again into 2 groups. One was treated with irbesartan [ 50 mg/（ kg·d ） , with stomach-tube ] , and the other received equal saline, so did the control group. After 12 weeks, the protein level of CT-1 was detected by immunohistochemistry. Results Ventricular CT-1 in the model control group and the treatment group was higher than that in the control group, and the correlation analysis showed that ventricular CT-1 of the model control group was positively correlated with the left ventricular weight index, and CT-1 of the treatment group was lower than that of the model control group. Conclusion CT-1 was assumed to take part in the ventricular remodeling. The mechanism of irbesartan on the ventricular remodeling may be related to the downregulation of CT- 1 expression.

关 键 词：心肌营养素-1 细胞因子 心室重构 

分 类 号：R541.6[医药卫生—心血管疾病]