黄芪对糖尿病大鼠肾组织血管生成素受体Tie-2表达的作用  

Effects of Astragalus on expression of renal angiopoietin receptor Tie-2 in diabetic rats

作　　者：顾红卫[1] 倪兆慧[1] 顾乐怡[1] 严玉澄[1] 戴慧莉[1] 李宁丽[2] 张敏芳[1] 钱家麒[1] 

机构地区：[1]上海交通大学医学院仁济医院肾脏科,上海200127 [2]上海交通大学医学院免疫研究所,上海200025

出　　处：《中西医结合学报》2007年第5期536-540,共5页Journal of Chinese Integrative Medicine

基　　金：上海市科学技术委员会基金(No.014319360)

摘　　要：目的:探讨血管生成素受体Tie-2在糖尿病大鼠肾组织中的表达以及黄芪对其影响。方法:SD大鼠随机分为正常对照组、糖尿病组和黄芪治疗组。8周后处死大鼠,采用实时定量聚合酶链反应和免疫组化法分别检测3组大鼠肾组织中Tie-2基因和蛋白表达水平。结果:与正常对照组相比,糖尿病组大鼠肾组织Tie-2蛋白表达显著升高(P<0.01);黄芪治疗组大鼠肾组织Tie-2蛋白表达较糖尿病组显著下调(P<0.01)。结论:黄芪通过下调糖尿病大鼠肾组织Tie-2的表达而发挥肾保护作用是其治疗糖尿病肾病的作用机制之一。Objective： To study the expression of angiopoietin receptor Tie-2 in the renal tissue of diabetic rats and the effects of Astragalus. Methods： SD rats were randomly divided into normal control group, diabetes group and Astragalus-treated group. The expression of receptor Tie-2 in the renal tissue was assessed by using real-time quantitative polymerase chain reaction and immunohistochemical method. Results： Glomerule Tie-2 protein expression was significantly elevated in the diabetes group as compared with the normal control group （P〈0.01）. Glomerule Tie-2 protein expression in the Astragalus-treated group was decreased as compared with the diabetes group （P〈0. 01）. Conclusion： Tie-2 may play an important role in the pathogenesis of the early stage diabetic renal injury. The reno-protection effect of Astragalus may be mediated by down-regulating the expression of Tie-2 in the kidney tissue of diabetic rats.

关 键 词：糖尿病肾病 血管生成因子 黄芪 大鼠 

分 类 号：R285.5[医药卫生—中药学]