黄连解毒汤对2型糖尿病大鼠靶组织磷脂酰肌醇3激酶的影响  

Effects of Huanglian Jiedu Decoction on phosphatidylinositol-3-kinase expression in target tissues of type 2 diabetic rats

作　　者：金丹[1] 陆付耳[1] 陈广[1] 孙焕[1] 陆小红 

机构地区：[1]华中科技大学同济医学院同济医院中西医结合研究所,湖北武汉430030 [2]湖北天茂集团制剂厂,湖北荆门448000

出　　处：《中西医结合学报》2007年第5期541-545,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.30371816);高等学校博士学科点专项科研基金资助课题(No.20030487008)

摘　　要：目的:观察黄连解毒汤对2型糖尿病大鼠骨骼肌和脂肪组织磷脂酰肌醇3激酶(phosphatidylinositol-3- kinase,PI-3K)p85亚基mRNA及蛋白表达的影响,探讨该方治疗2型糖尿病的分子机制。方法:Wistar雄性大鼠以尾静脉注射30 mg/kg链脲佐菌素(streptozotocin,STZ)配合高脂高热量饮食喂养的方法建立2型糖尿病模型。然后将动物随机分为模型组、阿司匹林组和黄连解毒汤组,分别用药治疗。另设12只正常对照组,不做任何处理。10周后,检测空腹血糖(fasting blood glucose,FBG)及空腹血清胰岛素(fasting serum insulin,FINS),并进行葡萄糖耐量试验(oral glucose tolerance test,OGTT);采用RT- PCR检测骨骼肌和脂肪组织中胰岛素刺激前后PI-3K p85亚基的mRNA表达;用Western blotting方法检测其蛋白表达水平。结果:黄连解毒汤组FBG和OGTT各时点血糖水平明显低于模型组(P<0.05);其FINS值明显低于正常组(P<0.05),而与模型组相比,差异无统计学意义。黄连解毒汤组骨骼肌和脂肪组织中PI-3K mRNA表达水平和蛋白表达水平较模型组明显增加。结论:黄连解毒汤治疗2型糖尿病的作用可能与其提高2型糖尿病大鼠肌肉和脂肪组织中PI-3K p85亚基mRNA和蛋白表达有关。Objective： To observe the effects of Huanglian Jiedu Decoction （HLJDD）, a traditional Chinese compound herbal medicine, on p85 mRNA and protein expressions of phosphatidylinositol-3-kinase （PI-3K） in target tissues （skeletal muscular and adipose tissues） in rats with type 2 diabetes mellitus （T2DM） and to investigate the molecular mechanism of HLJDD in treating T2DM. Methods： The male Wistar rats were injected with streptozotocin （STZ） 30 mg/kg through tail vein, and fed with high-fat and high-caloric diets to induce T2DM. Then the rats were randomly divided into untreated group, aspirin-treated group and HLJDD group, and treated correspondingly. Meanwhile, a group of normal animals without any treatment was set up for normal control group. Ten weeks later, serum fasting blood glucose （FBG）, serum fasting insulin （FINS） and oral glucose tolerance test （OGTT） were routinely determined. The expressions of PI-3K p85 mRNA and protein in skeletal muscle and adipose tissue were determined with RT-PCR and Western blotting before and after insulin treatment. Results： Compared with the untreated group, the FBG and OGTT levels in T2DM rats treated with HLJDD decreased significantly （P〈0.05）. The FINS in HLJDD group was lower than that in the normal control group （P〈0. 05）, but was not significantly different from that in the untreated group. The PI-3K p85 mRNA and protein expressions in HLJDD group obviously increased, as compared with those in the untreated group. Conclusion： The effect of HLJDD in treating T2DM was probably associated with its improvement of PI-3K p85 mRNA and protein expressions in skeletal muscle and adipose tissue of the T2DM rats.

关 键 词：黄连解毒汤 2型糖尿病 磷脂酰肌醇3激酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]