宝宝乐口服液对厌食大鼠食欲中枢神经元兴奋性的影响  

Effects of Baobaole oral liquid on neuron excitability of feeding center in anorectic rats

作　　者：杜永平[1] 张月萍[2] 张国成[2] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032 [2]第四军医大学西京医院儿科,陕西西安710032

出　　处：《中西医结合学报》2007年第5期550-554,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.39670896和No.30271634)

摘　　要：目的:观察宝宝乐口服液对厌食大鼠摄食中枢下丘脑外侧区(lateral hypothalamic area,LHA)和饱中枢下丘脑腹内侧核(ventromedial hypothalamic nuclear,VMN)神经元兴奋性的影响。方法:特制饲料喂养幼龄大鼠1周,成功制备厌食大鼠模型,运脾复方宝宝乐口服液灌胃3周,然后采用细胞外记录法,记录大鼠LHA和VMN神经元的自发放电,观察其对电剌激胃迷走神经和静脉注射葡萄糖的反应,比较正常组、模型组和治疗组间的差异。结果:模型组大鼠LHA神经元对胃迷走神经刺激的反应特征与正常组差异无统计学意义,而VMN神经元对胃迷走神经刺激的兴奋性反应时程延长(P<0.01),刺激强度降低(P<0.01)。模型组大鼠对胃迷走神经刺激有反应的LHA神经元的血糖敏感率降低,而那些对胃迷走神经刺激有反应的VMN神经元的血糖敏感率增加(P<0.01);治疗组大鼠LHA和VMN神经元对胃迷走神经刺激和静脉注射葡萄糖的反应与正常组差异无统计学意义。结论:宝宝乐口服液能调节LHA和VMN神经元对外周传入的摄食负反馈信号的敏感性,使LHA和VMN神经元共同作用,发出适度摄食信号,达到促进食欲的治疗目的。Objective： To investigate the effects of Baobaole oral liquid on neuronal excitability in lateral hypothalamic area （LHA） and ventromedial hypothalamic nuclear （VMN） in anorectic rats. Methods： The anorectic rat model was established by feeding with special prepared forage for a week, and then Baobaole oral liquid, a liquid extract of a compound traditional Chinese medicine for activating spleen, was administered once a day for 3 weeks. Finally, extracellular recording from LHA and VMN neurons in rats were made in order to characterize their responses to gastric vagal nerve stimulation and intravenous injection ofglucose in the normal, untreated, and Baobaole-treated groups. Results： There was no statistical difference in response characteristics of LHA neurons to gastric vagal stimulation among 3 groups. The duration of VMN neuron excitation response to gastric vagal nerve stimulation in the untreated group was significantly longer than that of the normal control group （P〈0. 01）, while the required stimulation intensity was significantly decreased （P〈0. 01）. Moreover, among the neurons responding to the gastric vagal stimulation in the untreated group, the number of glycemiaensitive neurons decreased in LHA and increased in VMN （P〈0. 01）. The gastric vagal stimulation induced neuron responses in LHA and VMN of the Baobaole-treated group were not significantly changed as compared with the normal control group （P〈0. 01）, and neither were the intravenous injection of glucose induced responses. Conclusion： Baobaole oral liquid can modulate the sensitivity of LHA and VMN neurons to the peripheral signal and make the coordination between LHA and VMN neurons in order to improve the appetite of anorectic rats.

关 键 词：中药 厌食 食欲调节 下丘脑外侧区 下丘脑腹内侧核 迷走神经 大鼠 

分 类 号：R285[医药卫生—中药学]