“脾虚证”大鼠下丘脑及结肠组织生长抑素和胆囊收缩素-8含量的变化  

Content of somatostatin and cholecystokinin-8 in hypothalamus and colons in a rat model of spleen-deficiency syndrome

作　　者：刘群[1] 蔡淦[2] 

机构地区：[1]第二军医大学长海医院中医科,上海200433 [2]上海中医药大学曙光医院消化科,上海200021

出　　处：《中西医结合学报》2007年第5期555-558,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金(No.30171124)

摘　　要：目的:观察脾虚证大鼠神经肽含量的变化。方法:大鼠随机分为3组:正常组、模型组和治疗组,每组各10只。采用大黄水煎浓缩剂灌胃建立脾虚证模型,治疗组予四君子汤灌胃治疗;正常组和模型组予生理盐水灌胃。采用放射免疫法测定大鼠下丘脑及结肠组织中生长抑素(somatostatin,SS)和胆囊收缩素-8(cholecystokinin-8,CCK-8)含量;同时测定尿D-木糖(D-xylose)含量。结果:模型组大鼠下丘脑CCK-8含量高于正常组,差异有统计学意义(P<0.05);模型组大鼠结肠黏膜SS含量及CCK-8含量高于正常组,差异均有统计学意义(P<0.05);模型组大鼠结肠黏膜CCK-8含量低于治疗组,差异亦有统计学意义(P<0.05);模型组大鼠尿D-木糖排泄率低于正常组,差异有统计学意义(P<0.05)。结论:脾虚证大鼠神经内分泌调节的紊乱可能是"脾虚-脾失健运"发生发展的重要基础。Objective： To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome. Methods： Rats were randomly divided into three groups： normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups, The somatostatin （SS） and cholecystokinin-8 （CCK-8） content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected. Results： The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group （P〈0.05）, SS and CCK-8 content in colons of the untreated group was higher than that of the normal group （P〈0.05）. The CCK-8 content in colons of the untreated group was lower than that of the treatment group （P〈0.05）. The excretion rate of D-xylose in the untreated group was lower than that of the normal group （P〈0.05）, Conclusion： The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for “spleen-deficency and dysfunction of spleen” in traditional Chinese medicine,

关 键 词：脾虚 神经肽类 下丘脑 结肠 大鼠 

分 类 号：R256.3[医药卫生—中医内科学]