竹节参总皂苷对人白血病细胞株HL-60的作用  

Effects of total saponins of Panax japonicus on human leukemic HL-60 cells

作　　者：袁丁[1] 左锐[1] 张长城[1] 

机构地区：[1]三峡大学医学院

出　　处：《中西医结合学报》2007年第5期570-572,共3页Journal of Chinese Integrative Medicine

基　　金：湖北省教育厅基金(No.2003A007);湖北省卫生厅基金(No.SDZZ2004059)

摘　　要：目的:研究竹节参总皂苷(total saponins of Panax japonicus,TSPJ)对人白血病细胞HL-60的作用,探讨其作用机制,为进一步开发竹节参提供理论依据。方法:体外培养人早幼粒白血病细胞HL-60,细胞计数法观察TSPJ对细胞活力的影响,四唑氮蓝(nitroblue tetrazolium,NBT)测定其对细胞还原能力的影响,流式细胞仪检测细胞周期变化和膜表面分化抗原CD11b的表达情况。结果:和阴性对照组比较,不同浓度TSPJ处理的细胞活力显著下降,NBT阳性细胞增多,G_0/G_1期细胞增多,S期细胞减少,膜表面分化抗原CD11b的表达量增多。结论:TSPJ对体外培养的HL-60细胞生长育一定抑制作用,其作用机制与诱导细胞分化和周期阻滞有关。Objective： To study the effects of total saponins of Panax japonicus （TSPJ） on human leukemic HL-60 cells. Methods： Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium （NBT） was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. Results： Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G0/G1 phase in the different concentrations of TSPJ-treated groups were increased. Conclusion： TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.

关 键 词：竹节参 HL-60细胞 细胞分化 

分 类 号：R285.5[医药卫生—中药学]