异黄酮类植物雌激素通过雌激素受体对靶基因的转录调节作用  

Effect of isoflavones in regulating the transcription of target genes through estrogen receptors

作　　者：孟庆书[1] 朱晓燕[1] 唐晓露[1] 马蓓[1] 倪鑫[1] 

机构地区：[1]第二军医大学基础部生理学教研室,上海200433

出　　处：《中西医结合学报》2007年第5期577-580,共4页Journal of Chinese Integrative Medicine

基　　金：上海市科技发展基金(No.06jc14083)

摘　　要：目的:观察异黄酮类植物雌激素染料木素和大豆苷元通过雌激素受体(estrogen receptor,ER)对靶基因的转录调节作用。方法:采用磷酸钙瞬时转染的方法,利用转染雌激素受体表达质粒的Hela细胞,观察染料木素和大豆苷元对雌激素反应元件(estrogen response element,ERE)报告基因的转录激活作用;此外还观察ER拮抗剂ICI 182780对这两种植物雌激素转录激活作用的影响。结果:染料木素和大豆苷元均可通过ER的两种亚型ERα和ERβ激活ERE报告基因的转录,且这种转录激活作用能被ICI 182780所阻断。结论:染料木素和大豆苷元均能产生类雌激素样作用,通过ERα和ERβ激活ER靶基因的转录。Objective： To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes. Methods： In this study, we used ERα or ERβ over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation. Results： Our results showed that both genistein and daidzein could activate ERE receptor gene through ERα and ERβ, and these effects could be blocked by ICI 182780. Conclusion： Both genistein and daidzein can mimic estrogen＇s effect to activate the transcription of target genes through binding to the ERs.

关 键 词：异黄酮类 染料木素 大豆苷元 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]