DNA倍体分析在宫颈病变诊断中的价值  

Dignosis of DNA ploid analysis in cervical disease

作　　者：李建业[1] 朱淑霞 

机构地区：[1]河北医科大学第二医院,河北石家庄050000 [2]武警河北总队医院

出　　处：《山东医药》2007年第24期10-11,共2页Shandong Medical Journal

摘　　要：目的探讨宫颈细胞DNA倍体分析在宫颈病变诊断中的价值。方法对接受宫颈癌普查的妇女用宫颈刷取材,液基薄层制片,制片后剩余细胞行流式细胞术DNA倍体分析;对其中290例患者做了活检病理诊断,其中非典型鳞状上皮增生(ASCUS)120例、低度宫颈上皮内瘤变(LSIL)90例、高度宫颈上皮内瘤变(HSIL)60例、鳞状上皮癌(SCC)20例。结果DNA异倍体在ASCUS、LSIL、HSIL、SCC组中的检出率分别为3.3%、31%、93%、100%;LSIL、HSIL、SCC三组与ASCUS组相比,P均<0.05;HSIL、SCC二组与LSIL组相比,P均<0.05;ASCUS、LSIL、HSIL、SCC组中,非DNA整倍体细胞者分别有25%、85.7%、96%、100%发生了2级宫颈上皮内瘤样病变(CIN_2)及以上级别的病变。结论DNA倍体分析可以明显提高CIN_2及以上级别宫颈病变的检出率。[ Objective] To explore the the value of DNA ploid analysis in cervical disease. [ Methods] FCM was used to detect the cervical cells of 290 patients including atypical squamous cells of undetermined significance（ASCUS） 120 cases, low- grade squamous intraepithelial lesion（LSIL） 90 cases, high -grade squamous intraepithelial lesion（HSIL） 60 cases, squamous cell cancer （ SCC）20 cases. [ Results] The rate of DNA heteroploid was 3.3% in ASCUS group, 31% in LSIL group, 93% in HSIL group, 100% in SCC group. Heteroploid level of LSIL,HSIL in SCC group were different from that of ASCUS group（P 〈 0.05 ）. Heteroploid level of HSIL in SCC group were different from that of LSIL group（ P 〈 0.05）. The rate of CIN2 or above CIN2 were 25% ,85.7% ,96%, 100% in the the group of ASCUS,LSIL, HSIL,SCC with the heteroploid. [ Conclusion ] DNA ploid analysis can be successfully used to detect CIN2 or above CIN2 lesions.

关 键 词：宫颈病变 宫颈细胞 DNA倍体分析 

分 类 号：R711.74[医药卫生—妇产科学]