TGF-β_1基因转染对大鼠树突状细胞生物学特性的影响  

Effect of biological characteristics of dendritic cells transfected by transforming growth factor β_1 gene in rat

作　　者：李纪鹏[1] 黄怡[1] 王为忠[1] 董光龙[1] 杜建军[1] 陈冬利[1] 季刚[1] 刘骥[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院,陕西西安710032

出　　处：《山东医药》2007年第24期23-24,共2页Shandong Medical Journal

基　　金：国家自然科学基金(30571803)。

摘　　要：目的观察转化生长因子β_1(TGF-β_1)基因转染后对大鼠树突状细胞(DC)生物学特性的影响。方法将TGF-β_1基因导入大鼠DC,G418筛选,检测转染细胞TGF-β_1的表达及其生物学活性,并进行混合淋巴细胞反应(MLR)。结果TGF-β_1基因转染的DC可以分泌TGF-β_1,而且可特异性抑制血管内皮细胞,对T淋巴细胞的增殖有抑制作用。结论利用构建的TGF-β_1表达质粒转染大鼠DC,转入的TGF-β_1基因可以在DC内稳定表达,并且使DC的生物学特性发生相应的改变。[ Objective ] To investigate the effects of transforming growth factor β1 （TGF - β1 ）gene transfer on the biological characteristics of dendritic cell （DC） in rat. [ Methods] DCs were transfected with pAd - TGF - β1 plasmid through lipofectamine mediated gene transfer, and the transfected cells were selected by G418. The biological characteristics of transfected DCs were detected by Western - Blot, vascular endothelial cell growth - inhibition test and mixed lymphocyte reaction （MLR） . [ Results] The transfected cells could excrete TGF - β1 ,and the supernatant could inhibit the growth of mink lung epithelium cell and the proliferation of T lymphocyte cells. [ Conclusions ] DCs transfected by pAd - TGF - β1 can express TGF - β1 stably, and the biological characteristics of transfected DCs are changed corespondingly.

关 键 词：转化生长因子 树突状细胞 基因转染 

分 类 号：R392[医药卫生—免疫学]