丹红注射剂对大鼠脑梗死灶周突触和星形胶质细胞的影响  

Effect of astrocyte on synapse and motor skill after treating by the Danhong injection in rats with acute cerebral infarction

作　　者：谭来勋[1] 段申汉[1] 曾庆杏[1] 曾非[1] 孙圣刚[2] 

机构地区：[1]武汉大学人民医院神经科,湖北武汉430060 [2]华中科技大学同济医学院附属协和医院神经科

出　　处：《中国医师杂志》2007年第8期1009-1012,共4页Journal of Chinese Physician

基　　金：国家教育部高等学校博士点学科专项科研基金（N0.20020487065）

摘　　要：目的研究步长倍通-丹红注射剂治疗后大鼠脑梗死周边区星形胶质细胞、突触和运动功能的变化及相关性。方法将120只大鼠大脑中动脉闭塞脑梗死模型随机分为丹红、抑制、盐水和对照组，以横木行走试验评定运动功能，在5、10、15 d应用电镜和免疫组织化学方法观察各组大鼠脑梗死周边区星形胶质细胞、突触、胶质纤维酸性蛋白和突触素等的变化。结果大鼠运动功能10 d、15 d分别为丹红组（3.2±0.3）和（5.8±0.9）分，较对照组（1.8±0.5）和（2.6±0.8）分、抑制组（1.6±0.9）和（2.1±0.7）分高（P〈0.01）；电镜观察，10 d、15 d脑梗死周边区星形胶质细胞分别为（5.8±0.3）、（8.5±0.8）和突触数目（12.3±1.3）、（16.3±1.4）（个/八千倍视野）同步增多，两者均明显多于对照组和抑制组（P〈0.01）；免疫组化染色观察，脑梗死周边区胶质纤维酸性蛋白和突触素阳性信号密度值也在10 d、15 d高于对照组和抑制组（P〈0.01）。结论用步长倍通-丹红注射剂治疗可使脑梗死大鼠星形胶质细胞增殖，加强突触重建和功能修复，促进运动功能恢复；星形胶质细胞增殖可能在其中发挥关键性作用。Objective To investigate the relation between astrocytes and synapses in the region around the infarction area （area A） and the roles of the astrocytes on the recovery of motor skill in the experimental rats with cerebral infarction after treating by the Danhong injection of Buchang. Methods 120 rats were random divided into the groups of Danbong, inhibitation, saline and control. The motor skill was examined via beam walking test. The changes were observed about astrocytes, synapses, glial fibrillary acidic protein （GFAP） and synaptophysin in area A with electron microscope and immanohistochemistry at 5, 10 and 15d after the model was made. Results The motor skill mark was significantly higher in the Danbong group than that in the control or inhibitation. The synapse increased with the astrocytes in number gradually increased, which were significantly higher in the Danhong group than that in the control or inhabitation group. It had no significant difference of optical density between GFAP and synaptophysin in the Danbong group and the control or inhibitation group. However, a large difference of the optical density between these groups were observed at both 10d and 15d, with a higher value in Danbong group （ P 〈0. 01 ）. Conclusions Danbong injection of Buchang can induce motor skill recovery and increase the number and function of astrocyte and synapse in the rats with cerebral infarction, where the astrocyte may play a crucial role.

关 键 词：丹参/药理学 红花/药理学 脑梗塞 突触 星形细胞 注射剂 

分 类 号：R28[医药卫生—中药学]