高含量HBsAg致ELISA一步法假阴性的探讨  

Study on false negative of one step ELISA resulting from high level HBsAg

作　　者：赖雄 吴兆勇 余岸松[2] 

机构地区：[1]广东省湛江市第二人民医院检验科,广东湛江524003 [2]广东海洋大学医院,广东湛江524088

出　　处：《吉林医学》2007年第7期879-880,共2页Jilin Medical Journal

摘　　要：目的:探讨乙型肝炎表面抗原(HBsAg)含量过高致ELISA一步法钩状效应(hook effect)的产生及对策。方法:HBeAg阳性HBsAg阴性标本15例,倍比稀释标本后分别应用ELISA二步法和一步法检测HBsAg,观察两种方法的阳性率及不同稀释比与吸光度的变化。结果:检测15份标本原血清结果,一步法HBsAg均为阴性,二步法均为阳性;一步法,大部分标本从原血清至1:8均为阴性,吸光度值OD为0.075,1:16稀释后OD值逐渐升高,从1:128至1:1 024吸光度变化不大(OD:3.30±0.15),1:2 048后OD值逐渐下降;二步法从原血清到1:256吸光度变化不大(OD:3.20±0.18)),1:512后OD值逐渐下降。结论:ELISA二步法可避免HBsAg浓度过高致钩状效应所引起的HBsAg检测结果假阴性。Objective To study the reasons and countermeasures of hook effect in one step ELISA resulting from high level HBsAg. Method HBsAg of diluted specimens were detected with one step and two step ELISA among 15 blood specimens with HBeAg positive and HBsAg negative, in order to observe the change of positive rate and absorbance in different diluted specimens with the two methods. Resuits HBsAg was negative using one step, while using two step was positive in the detection of 15 original serum specimens. In one step ELISA, HBsAg in most specimens diluted from original serum to 1 ： 8 was negative and absorbance （OD） was 0. 075, then OD went up gradually after 1：6 dilution, from 1：128 to 1：1 024 dilution, OD had little changes （OD： 3.30 ± 0.15）, OD droped gradually after 1： 2 048 dilution ; in two step ELISA, from original serum to 1 ： 256 dilution, OD had little changes （ OD ： 3.20 ± 0.18 ） , OD droped gradually after 1：512 dilution. Conclusion The false negative of hook effect in one step ELISA resulting from high level HBsAg can be avoided by two step ELISA.

关 键 词：ELISA 乙型肝炎表面抗原 钩状效应 

分 类 号：R446.6[医药卫生—诊断学]