HPLC-ELSD法测定杏灵颗粒中萜类内酯的含量  

Determination of terpenelactones in Xingling Granules by HPLC-ELSD

作　　者：马凤仙[1] 邱金东[2] 李钦[2] 

机构地区：[1]河南大学成人教育学院,河南开封475001 [2]河南大学中药研究所,河南开封475004

出　　处：《河南大学学报（医学版）》2007年第3期25-27,共3页Journal of Henan University:Medical Science

摘　　要：目的:测定杏灵颗粒中萜类内酯的含量。方法:采用高效液相色谱法蒸发光散射检测器(HPLC-ELSD)测定杏灵颗粒中萜类内酯的含量。色谱条件为:Angilent Hypersil ODS(5μm,4 mm×250 mm)柱;四氢呋喃-甲醇-水(10∶20∶70)为流动相;蒸发光散射检测器检测参数:漂移管温度103℃;载气(N2)流速2.51 L/min。结果:该方法简便易行,杏灵颗粒中基质对萜类内酯的测定无干扰,线形关系良好;萜类内酯的重现性试验RSD为1.63%;平均加样回收率为96.8%。结论:本方法可作为杏灵颗粒中萜类内酯成分质量控制的一种有效方法。Objective： To determine the content of terpenelactonesines in Xingling Granules. Methods： HPLC with Evaporative Light Scattering Detector（ELSD） was established to determine the content of terpenelactones in Xingling Granules. The analysis was carried out on Angilent Hypersil ODS（5 μm, 4 mm × 250 mm） column. The mobile phase consisted of THF-MeOH-H2O（ 10： 20： 70） . The operating conditions of the ELSD were a nebulizer nitrogen flow-rate of 2.51 L/min and an evaporator tube temperature of 103 ℃. Results： No interference was found in the terpenelactones peak position. The method was simple, with a good linear relationship. The relative standard deviation of reproducibility was 1.63 % and the average recovery was 96.8 %. Conclusion： The method can he used for quality control.

关 键 词：杏灵颗粒 萜类内酯 高效液相色谱 蒸发光散射检测器 

分 类 号：R927.3[医药卫生—药学]