卡维地洛对心肌梗死后心房缝隙连接重构的影响  

Effects of Carvedilol on Atrial Gap Junctional Remodeling After Chronic Left Ventricular Myocardial Infarction

作　　者：曹锋[1] 黄从新[1] 江洪[1] 李夏[1] 包明威[1] 唐艳红[1] 

机构地区：[1]武汉大学人民医院心血管内科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2007年第5期552-555,I0001,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30470704)

摘　　要：目的:研究慢性左室心肌梗死对心房肌缝隙连接蛋白重构的影响,并观察肾上腺素能受体阻断剂卡维地洛的干预作用。方法:新西兰白兔30只随机分为3组:对照组10只,心肌梗死组10只,卡维地洛干预组10只。心肌梗死组结扎冠状动脉左前降支;对照组只开胸但不结扎冠状动脉。卡维地洛5 mg/(kg.d)组直接灌胃给药,另外两组灌等量蒸馏水,3组均喂养8周。8周后采用免疫荧光组织化学法观察左心耳缝隙连接蛋白40(Cx40)的空间分布,Western blot技术检测左心耳Cx40的含量变化。结果:心肌梗死组左心耳Cx40的含量与对照组相比显著降低(P<0.01),并呈现不均一分布;卡维地洛干预组Cx40的含量比心肌梗死组高(P<0.05),不均一分布程度减轻。结论:慢性左室心肌梗死可使心房Cx40发生重构,其分布高度异质性,且蛋白含量明显下降,卡维地洛可减轻Cx40的重构。Objective： To investigate the effects of carvedilol on atrial gap junctional remodeling in a rabbit model of chronic left ventricular myocardial infarction （MI）. Methods： Thirty rabbits were randomly divided into three groups： control group （n= 10, thoracotomy, left anterior descending artery was not ligated） and MI （n= 10, thoracotomy ＋ MI, left anterior descending artery was ligated） group, and Carvedilol group （n= 10）. The rabbits in the carvedilol group received oral carvedilol [5 mg/（kg·d）] for eight weeks. The rabbits in the other two groups received a matching saline. Eight weeks after operation, distribution of atrial connexin 40（Cx40） and its protein level were studied by immunohistochemistry and Western blot. Results： In MI group, atrial Cx40 level of left atrial appendage decreased significantly than that in control group （P〈0.01）. The distribution Cx40 was changed. The side-to-side junctions increased, while the end-to-end junctions decreased. Atrial Cx40 in MI group was highly heterogeneous. In the Carvedilol group, the atrial Cx40 level increased significantly than that in MI group （P%0.05）. Conclusion： Chronic left ventricular myocardial infarction can results in atrial Cx40 remodeling. Carvedilol can attenuate the Cx40 remodeling effectively.

关 键 词：心肌梗死 缝隙连接 心房颤动 卡维地洛 

分 类 号：R542.22[医药卫生—心血管疾病] R541.7[医药卫生—内科学]