白细胞介素-18、-6在糖尿病大鼠肾脏的表达  

Expression of Interleukin-18 and Interleukin-6 in the Renal of Diabetic Rats

作　　者：付秀立[1] 李竞[1] 

机构地区：[1]武汉大学人民医院内分泌科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2007年第5期602-604,I0003,共4页Medical Journal of Wuhan University

摘　　要：目的:检测白细胞介素(IL)-18、-6在糖尿病大鼠肾脏的表达,探讨其在糖尿病肾脏病变过程中的作用。方法:40只雄性Wistar大鼠,随机分为正常对照4周(NC1组)及8周(NC2组),糖尿病4周(DM1组)及8周(DM2组),每组10只。DM组按60 mg/kg一次性腹腔内注射链脲佐菌素建立糖尿病大鼠模型。检测各组大鼠体重(BW)、肾重、尿量(UV)及尿微量白蛋白排泄率(UAER)。用免疫组化方法检测各组肾组织IL-18、IL-6的表达,利用计算机图像分析系统进行定量分析。结果:DM组大鼠的肾重指数(KWI)、UV、UAER较NC组大鼠显著增高(P<0.01),DM2组较DM1组增高明显(P<0.05)。IL-18、IL-6在NC组大鼠肾组织中仅有少量表达,DM1组表达较NC组明显增多(P<0.01),DM2组表达增高更为显著(P<0.01)。肾脏局部IL-18、IL-6与UAER呈显著正相关(r=0.471,P<0.01,r=0.513,P<0.01)。结论:炎性反应在糖尿病肾脏局部损害中发挥重要的作用。Objective： To investigate the potential role of IL-18 and IL-6 expression in the renal of diabetic rats. Methods- Forty male Wistar rats were divided at random and equally into normal control group of 4 weeks （NC1 group） and 8 weeks （NC2 group）, diabetic mellitus group of 4 weeks （DM1 group） and 8 weeks （DM2 group）. The rat models in DM group were induced by injection of streptozotocin （STZ, 60 mg/kg, intra-peritoneally）. The expression of IL-18 and IL-6 in the renal tissue was detected by immunohistochemistry staining and computer image-pattern analysis system. At the same time, kidney weight index（KWI）, urine volume（UV） and UAER were measured. Results： The levels of KWI, UV and UAER were significantly higher in DM groups （all P〈0.01）. The expression of IL-18 and IL-6 was weaker in NC group, while stronger in DM1 group （P〈0.01） and much stronger in DM2 group （P〈0.01）. There was a significant positive correlation between the expression of IL-18/IL-6 and UAER （r=0. 462, P〈0.01 and r =0. 513, P〈0.01, respectively）. Conclusion： Inflammation factors play an important role in the progression of renal lesion in diabetic rats.

关 键 词：糖尿病 糖尿病肾病 白细胞介素 

分 类 号：R587.2[医药卫生—内分泌]