Nafion修饰分散铋电极线性扫描伏安法测定肝素钠含量  

Linear sweep voltammetric determination of heparin sodium with a Nafion-modified dispersed bismuth particle electrode

作　　者：杜美菊[1] 黄华伟[1] 

机构地区：[1]商丘师范学院化学系,河南商丘476000

出　　处：《化学试剂》2007年第9期535-536,576,共3页Chemical Reagents

基　　金：河南省自然科学基金资助项目(0511020300)

摘　　要：利用循环伏安法（CV）、线性扫描伏安法（LSV）研究了肝素钠在铋膜玻碳电极上的电化学行为。在pH 5.4的B-R缓冲溶液中,肝素钠在-0.79 V左右处产生一灵敏的不可逆氧化峰,并对其电化学检测条件进行了优化,在选定的最佳条件下,峰电流与肝素钠浓度在0.4～2.0 mg/L范围与其峰高有良好的线性关系。回归方程为Ip=11.12＋80.91c（mg/L）,r=0.991,检测限为0.08 mg/L。考察了干扰物质的影响,据此建立了一种快速、简便测定肝素钠的方法。将本方法应用于肝素钠样品的测定,结果令人满意。The electrochemical behavior of heparin（Hep） is studied by circulation voltammetry（CV） and linear sweep voltammetry（LSV） with a bismuth film electrode.Hep gives a well-defined oxidation peak at-0.79 V in a BR buffer solution at a pH value of 5.4.Peak currents showed an excellent linear relationship with the Hep concentration ranging from 0.4～2.0 mg/L with the regression equation equating to Ip=11.12＋80.91c（mg/L）.The detection limit was 0.08 mg/L.The influence of interference substances on the test results was also investigated.Our method for the determination of Hep was rapid and simple and the results for analyzing the Hep samples was satisfactory.

关 键 词：Nafion修饰分散铋电极 肝素钠 循环伏安法 线性扫描伏安法 

分 类 号：O657.1[理学—分析化学]