苦皮素A标准品的制备研究  

Study on Preparation of Standard Substance Angulatin A

作　　者：卢桂荣[1] 吴鸣建[1] 刘金盾[1] 赵天增[2] 李倩[2] 

机构地区：[1]郑州大学化工学院,郑州450002 [2]河南省科学院天然产物重点实验室,郑州450002

出　　处：《河南科学》2007年第5期738-740,共3页Henan Science

基　　金：河南省自然科学基金项目(511022800)

摘　　要：为测定苦皮藤素产品中苦皮素A的含量,以控制苦皮藤素产品的质量,将苦皮藤根皮的95%乙醇提取物用硅胶柱分离、活性碳脱色后,利用制备高效液相色谱法制得苦皮素A的标准品,纯度达98.5%.制备型色谱柱为Shimpack PREP-ODS(20 mm×250 mm,15μm;)流动相为甲醇-水(体积比为703∶0)溶液;流速为15 ml/min;检测波长为232 nm;柱温为25℃.用13CNMR鉴定苦皮素A的结构.此方法得到的苦皮素A产品的纯度高,可用作分析方法的对照品.　In order to determine the content of angulatin A in angulatin products and control the quality of products,angulatin A was prepared with a series of steps,including extraction with 95 % ethanol,segregation by silica gel column,decolourization with active carbon,and separation with preparative high performance liquid chromatography.The purity of angulatin A was 98.5 %.Preparative chromatographic column Shimpack PREP-ODS（20 mm × 250 mm,15 μm）was used at 25 ℃ with methanol-water（70 ∶ 30,V / V）as mobile phase at a flow rate of 15 ml / min.UV detection wavelength was set at 232 nm.The chemical structure of angulatin A was identified by 13 CNMR.The purity of angulatin A obtained by this method was high and can be used as a reference substance.

关 键 词：制备高效液相色谱法 苦皮素A 苦皮藤素 苦皮藤 

分 类 号：O629.9[理学—有机化学] S482.1[理学—化学]