大鼠牙囊细胞培养方法的探讨  

Exploration of Culture Method of Rat Dental Follicle Cells In Vitro

作　　者：谷海晶[1] 凌均棨[1] 杜宇[1] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院牙体牙髓科,广东广州510055

出　　处：《中山大学学报（医学科学版）》2007年第5期586-589,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30672318);广东省卫生厅科技攻关项目(Z2005189)

摘　　要：【目的】探讨大鼠牙囊细胞体外培养的方法。【方法】分离出大鼠牙囊组织,分别采用组织块培养法、细胞消化分散法、及改良组织块法进行牙囊细胞原代及传代培养,倒置相差显微镜下进行细胞形态学观察,透射电镜观察细胞超微结构。【结果】培养至第3天,倒置相差显微镜观察发现,采用组织块培养法,见少数细胞从组织块爬出;而细胞消化分散法则可获得数量多的牙囊细胞,但与杂质细胞混杂生长;改良组织块法见量多、相对纯的牙囊细胞。电镜下牙囊细胞无桥粒,胞浆中含有高密度电子颗粒和大量粗面内质网。组织块法、细胞消化分散法和改良组织块法牙囊细胞培养成功率分别为80%、60%和100%;分别于10 d、7 d和6 d左右进行第一次传代。【结论】改良组织块法是一种良好的大鼠牙囊细胞培养方法。[Objective] To explore the culture method of rat dental follicle cells in vitro. [Methods] The dental follicle （DF） tissues were dissected for primary generation and passage culture by methods of explant, dissociation cell and improved explant, respectively. Consequently, the morphology of the cultured first passage DF cells and their uhrastructure were discovered under inversion phase difference microscope and transmission electronic microscope, respectively. [Results] At the third day in vitro, only a few cells extended from the explant by way of explant, and many DF cells mixing with impure cells adhered wall by way of dissociation cell. However, a large quantity of relative pure DF cells was discovered by way of improved explant at the same time. Transmission electron microscopy showed that the cultured cells contained electron-dense granules, and abundant rough endoplasmic reticulum（RER） and no desmosmoes. At about lOth, 7th and 6th day in vitro, DF cells were passed first time with achievement ratio of eighty, sixty and one hundred percent by ways of explant, dissociation cell and improved explant culture respectively. [ Conclusion ] Improved explant culture was a good culturing method for rat DF cells.

关 键 词：牙囊细胞 细胞培养 方法学 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]